Current Issue http://47.93.137.184/wjyx EN-US http://47.93.137.184/wjyx/EN/current.shtml http://47.93.137.184/wjyx 5 Objective To explore the key points of pharmaceutical care for patients after splenectomy under different surgical methods, and to provide useful reference information for clinical practice. Methods Based on the differences of the range of organ resection, reconstruction methods of the digestive tract, and incidence of postoperative complications of different pancreatic surgical methods, Clinical pharmacists selected three typical patients who had pancreatic fistula, abdominal infection, and blood glucose disorders after pancreaticoduodenectomy, distal pancreatectomy, and total pancreatectomy. By monitoring the amylase and drainage volume of the drainage fluid, postoperative pancreatic fistula could be treated. Attention was paid to the changes in various infection indicators and microbial culture results to adjust the anti-infection plan. Postoperative blood glucose changes were monitored and appropriate medication was given in a timely manner. The pharmaceutical care practice was combined with relevant literature to extract and analyze the key points of postoperative care under different surgical methods by combining. Results After pharmaceutical care by clinical pharmacists, the patient’s postoperative complications were controlled, and they were discharged in good condition. Conclusions Clinical pharmacists should should care patients after pancreatic surgery based on the surgical methods to improve the effectiveness and safety of treatment.]]> Objective To investigate the therapeutic effects of Yupingfeng granule combined with diphenhydramine attached umbilical in children with atopic dermatitis（AD）. Methods A total of 200 pediatric patients diagnosed with AD in Henan Provincial Corps Hospital of Chinese People’s Armed Police Force from March 2022 to May 2024 were randomly divided into two groups, with 100 cases in each group. The control group was given conventional treatment, while the treatment group was treated with Yupingfeng granules combined with diphenhydramine attached umbilical on the basis of the control group. The clinical efficacy,diphenhydramine on eosinophils（EOS）, vitamin D levels and adverse reactions before and after treatment were compared between the two groups. Results After treatment,the Eczema Area and Severity Index （EASI） and Scoring Atopic Dermatitis （SCORAD） scores markedly decreased in the treatment group versus the control group （P<0.05）.EOS level of the treatment group was significantly lower than that of the control group, and the vitamin D level was significantly higher than that of the control group（P<0.05）. There was no statistically significant difference in the incidence of adverse reactions between the two groups （P > 0.05）; the total effective rate of clinical efficacy in the treatment group was higher than that in the control group（97.00 % vs. 82.00%; P<0.05）. Conclusions Yupingfeng feng granules combined with diphenhydramine attached umbilical can reduce EOS level,increase vitamin D level and improve clinical efficacy in children with AD patients, with high safety.]]> Objective To compare the therapeutic effects of one-time radical surgery combined with new dressings and traditional surgical methods in treating perianal abscess in young patients. Methods Fifty patients with perianal abscess admitted to Hubei Provincial Corps Hospital of Chinese People’s Armed Police Force from January 2023 to August 2024 were selected and randomly divided into an observation group （one-time radical surgery+new dressing） and a control group （traditional incision and drainage + vaseline gauze）,with 25 patients in each group. The wound healing time,number of dressing changes,pain score （assessed by VAS）, and the incidence of anal fistula were compared between the two groups. Results The average healing time of the observation group and the control group were [（13.36±6.26）d vs.（21.56±9.21）d,P<0.05], the number of dressing changes was (7.96±2.42) times compared to （15.40±6.33） times （P<0.05）, the pain score at 1 week after surgery were [（4.64±1.19）vs.（6.00±1.22）,P<0.05] in the observation group and the control group, and the incidence of anal fistula at 6 months after surgery were 8% vs 36% （P<0.01）, all of which showed statistically significant differences. Conclusions Combination therapy can significantly shorten wound healing time,reduce dressing changes, alleviate pain, and lower the incidence of anal fistula, especially suitable for the rapid recovery needs of young people.]]> Objective To investigate the effect of antigen retrieval time and pH value of the retrieval solution on the immunohistochemical staining results of Pax-8 and CD10. Methods Fifteen surgical specimens of kidneys and livers （15 cases respectively） were selected,and the EnVision two-step immunohistochemical method was used to perform antigen retrieval with pH8.0 and pH9.0 in renal tissue Pax-8 and liver tissue CD10, respectively. The retrieval time was 1.5 min,2 min,2.5 min,and 3 min. The staining was evaluated by the main pathologist in a blind review. Results The positive expression of Pax-8 and CD10 in both groups of retrieval solutions was good（P< 0.05）. The double-factor analysis of variance of renal tissue Pax-8 antibody staining showed that the main effect of retrieval solution pH was significant（P< 0.01, partial η²= 0.424）. Backgroundstaining in pH 8.0 retrieval solution groups of 1.5 min, 2 min, 2.5 min, and 3 min was significantly lighter than that in pH 9.0 groups,with statistically significant difference （P< 0.01）. There was no statistically significant difference in the interaction effect of the retrieval time and the interaction effect between the two factors. For hepatic tissue CD10 antibody staining,double-factor ANOVA indicated a significant main effect of pH （P< 0.01, partial η²= 0.076）, non-significant main effect of retrieval r time （P> 0.05）, but significant interaction effect （P< 0.05, partial η²= 0.068）. The pH 8.0-1.5 min group showed significantly lighter background staining than pH 9.0-1.5 min group （P< 0.01）. Within pH 8.0 groups,no significant differences were observed across time points （P> 0.05）. Under pH 9.0 conditions, background staining progressively decreased with extended repair time, reaching the lightest level in 3-min group. Conclusions The background of Pax-8 staining n renal tissue is not related to antigen retrieval time,while pH 8.0 retrieval solution can reduce the background staining. For hepatic tissue CD10 staining,antigen retrieval duration should be appropriately adjusted according to pH variation of retrieval solutions：1.5 min for pH 8.0 and 3 min for pH 9.0.]]> Objective To analyze the distribution of pathogenic bacteria and their drug resistance in patients with ocular infection in a tertiary hospital in Beijing, and to provide reference for the clinical rational use of antibiotics. Methods A retrospective analysis was conducted to statistically analyze the composition and drug resistance of specimens submitted by patients with ocular infection in a tertiary hospital in Beijing from January 2021 to December 2023. Results A total of 255 pathogenic strains were isolated, with a positive rate of 56.42%. Among them, 134 （52.55%） strains of Gram-positive bacilli, 71 （27.84%） strains of Gram-negative bacilli, 39 （15.29%） strains of Gram-positive bacilli, and 11 （4.31%） strains of fungi were detected. The top 5 pathogenic bacteria isolations were Staphylococcus aureus, Staphylococcus epidermidis, Corynebacterium, Pseudomonas aeruginosa, and Streptococcus viridans, accounting for 18.04%, 14.09%, 10.20%, 7.45%, and 7.06%, respectively. The results of drug susceptibility tests showed that Gram-positive cocci had the highest resistance rate to penicillin and erythromycin, and the lowest resistance rate to vancomycin and linezolid. Enterobacteriaceae had lower resistance rate to levofloxacin and ciprofloxacin, at 28.00% and 24.00%, respectively. The overall resistance rate of Pseudomonas aeruginosa was relatively low. Conclusions The isolated bacteria from patients with ocular infection are mainly staphylococci, with Staphylococcus aureus being the most common one. Gram-positive bacteria are generally resistant to penicillin and erythromycin, but have lower resistance rate to rifampicin,and can be a better choice in clinically practice. The resistance rate of Gram-negative bacteria is generally low, and quinolones and aminoglycosides can be a choice in empirical practice.]]> Objective To analyze the expression and clinical significance of long non-coding RNA SBF2-AS1 （LncRNA SBF2-AS1） in hepatocellular carcinoma （HCC） tissues, and to explore its application value in theranostics of HCC. Methods A total of 54 patients with primary HCC admitted to the First Affiliated Hospital of Guangxi Medical University from 2021 to 2022 were selected as the study subjects. Postoperatively resected specimens from HCC and adjacent tissues were collected, along with multiple clinical treatment data, including pathological tissues, clinical laboratory diagnostics and treatment methods. The expression level of LncRNA SBF2-AS1 in the tissue specimens were detected by real-time quantitative polymerase chain reaction （RT-qPCR） and analyzed for correlation with clinical diagnostic and therapeutic indicators using biostatistical methods. Bioinformatics databases were utilized to analyze the diagnostic value of LncRNA SBF2-AS1 for HCC. Results The relative expression level of LncRNA SBF2-AS1 in HCC tissues （10.656） was significantly higher than that in adjacent tissues （1.000）, with a statistically significant difference （Z=-5.675,P<0.001）. The expression of LncRNA SBF2-AS1 was positively correlated with glutathione aminotransferase （r=0.297, P<0.05）, neutrophil （r=0.275, P<0.05）, and different treatment methods （r=0.332, P<0.05）. Patients were divided into a high expression group （27 patients） and a low expression group （27 patients） based on the median value of LncRNA SBF2-AS1 expression（M=3.649）, and the expression level of LncRNA SBF2-AS1 was correlated with ghrelin transaminase （P< 0.05）. The relative expression levels of LncRNA SBF2-AS1 in 371 cases of l HCC tissue and 50 cases of adjacent tissues were obtained from the cancer genome atlas （TCGA） database. The area under the ROC curve （AUC） was 0.9122, with specificity of 94.00% and sensitivity of 79.51%. Conclusions LncRNA SBF2-AS1 is highly expressed in HCC tissues, which is related to alanine aminotransferase, neutrophils and treatment methods, and can serve as a biomarker for HCC diagnosis and inflammatory injury monitoring, demonstrating significant application value in clinical theranostics.]]> Objective To investigate the predictive value of serum D-dimer （D-D）, troponin T （TNT） and B-type brain natriuretic peptide precursor （pro-BNP） in elderly patients with acute cerebral infarction accompanied by cerebrocardiac syndrome. Methods A total of 146 elderly patients with acute cerebral infarction from January 2022 to December 2023 were selected and divided into a cerebrocardiac syndrome group （56 cases） and non-cerebrocardiac syndrome group （90 cases） according to whether they developed cerebrocardiac syndrome. The baseline data and relevant laboratory examination indicators of the two groups were compared. Risk factors of cerebrocardiac syndrome in the patients were analyzed by multivariate Logistic regression,and the predictive value of D-D, TNT and pro-BNP was analyzed by ROC curve. Results Compared with non-cerebrocardiac syndrome group, the age, incidence of hypertension, incidence of diabetes, D-D, TNT, CK-MB and pro-BNP levels in the cerebrocardiac syndrome group were significantly higherr, with statistically significant differences （P<0.05）. Multivariate Logistic regression analysis showed that age, hypertension, D-D,TNT and pro-BNP were independent risk factors for the occurrence of cerebrocardiac syndrome in elderly patients with acute cerebral infarction. Using 2.05mg/L as the critical value of serum D-D,the area under ROC curve was 0.717, the predicted sensitivity was 68.7%, and the specificity was 87.2%. Using38.41ng/L as the critical value of serum TNT,the area under ROC curve was 0.799,the sensitivity was 90.5%, and the specificity was 60.3%. Taking 1268.36pg/mL as the critical value of serum pro-BNP, the area under ROC curve was 0.743, the sensitivity was 81.3%, and the specificity was 62.7%. The area under the combined ROC curve was 0.825, the sensitivity was 72.0%,and the specificity was 83.3%. Conclusions Elevated levels of serum D-D, TNT and pro-BNP are one of the risk factors of acute cerebral infarction complicated with cerebrocardiac syndrome in the elderly,which are characterized by predictive value for the occurrence of cerebrocardiac syndrome.]]> Objective To study the relationship between the expression levels of costimulatory molecule B7 homolog 4 （B7-H4）, nerve growth factor （NGF）, human epididymis protein 4 （HE4）, and tyrosine kinase （TrkA） and the progression and prognosis of cervical cancer. Methods A total of 102 patients who underwent radical hysterectomy for cervical cancer at General Hospital of PLA Northern Theater Command from January 2019 to December 2022 were selected, and their tumor tissues removed during the operation were used as samples. The expression of B7-H4, NGF, HE4 and TrkA was detected by immunohistochemistry. The expression levels of B7-H4, NGF, HE4 and TrkA in cervical tissues of patients with different FIGO stages, tumor grades, and whether accompanied by lymph node metastasis was compared. All the patients were followed up until June 2024, and the differences in the expression of B7-H4, NGF, HE4 and TrkA in cervical tissues between the dead and surviving patients were compared. Results Among the 102 patients, 19 were stage IIb, 29 were stage IIa, 31 were stage Ib, and 23 were stage Ia. Expression levels of B7-H4, NGF, TrkA and HE4 differed significantly across the different FIGO stages （P< 0.05）.Histologic grading was G1 in 27 cases, G2 in 50 cases and G3 in 25 cases. Expression levels of B7-H4, NGF, TrkA and HE4 varied significantly among the different tumour grades （P < 0.05）.Depth of cervical stromal invasion was ≤ 1/2 in 59 patients and > 1/2 in 43 patients. Expression levels of B7-H4, NGF, TrkA and HE4 were significantly higher in patients with invasion > 1/2 than in those with invasion ≤ 1/2 （P < 0.05）.Lymph-node metastasis was absent in 61 patients and present in 41 patients. Expression levels of B7-H4, NGF, TrkA and HE4 were significantly higher in patients with lymph-node metastasis than in those without （P < 0.05）.All patients were followed up until December 2023 （mean follow-up, 62.4 months）. At the last follow-up, 58 patients （56.86 %） were alive and 44 （43.14 %） had died. Expression levels of B7-H4, NGF, TrkA and HE4 were significantly different between the survival and death groups （P < 0.05）.ROC curves were constructed to evaluate the prognostic performance of each biomarker. The area under the curve （AUC） with 95 % confidence interval （CI） was 0.782 （0.696-0.889） for B7-H4, 0.741 （0.637-0.846） for NGF, 0.625 （0.506-0.744） for HE4 and 0.797 （0.696-0.898） for TrkA, indicating that each marker independently predicted prognosis. The combined model yielded an AUC （95 % CI） of 0.919 （0.856-0.982）, superior to that of any single marker alone. Conclusion B7-H4, NGF, HE4, and TrkA have certain value in predicting the prognosis of cervical cancer patients. Combined prediction can improve the sensitivity and specificity of prediction and can be used as molecular markers for predicting disease progression and prognosis and potential therapeutic targets.]]> Objective To validate the synergistic effect of lower limb rehabilitation robot combined with blood flow restriction training in the early rehabilitation after anterior cruciate ligament reconstruction. Methods A total of 90 young men who underwent rehabilitation after anterior cruciate ligament reconstruction in DaLian Rehabilitation Recuperation Center of PLA Joint Logistics Support Force from January 2024 to December 2024 were randomly divided into a robot group,a blood flow restriction group, and a combined group,with 30 cases in each group. All the three groups received routine rehabilitation training within 2 weeks after surgery. From three to six weeks after surgery,different interventions were added: the robot group received lower limb rehabilitation robot training,the blood flow restriction group received blood flow restriction training,and the combination group received lower limb rehabilitation robot combined with blood flow restriction training. The difference in thigh circumference,peak torque of the knee extensors,average stride length,average stride frequency, total length of the center of gravity movement trajectory,Lysholm score and the incidence of adverse events during rehabilitation were compared between the three groups after the intervention. Results After the intervention,the difference in thigh circumference and the total length of the center of gravity movement trajectory among the three groups were significantly lower than those before the intervention,and the peak torque of the knee extensors,average step length,average step frequency,and Lysholm score were significantly higher than those before the intervention（all P<0.05）. After the intervention,the difference in thigh circumference in the combined group and the blood flow restriction group was significantly smaller than that in the robot group （P<0.05）, and the combined group was significantly smaller than the blood flow restriction group （P<0.05）. The peak torque of the knee extensors in the combined group and the blood flow restriction group was significantly higher than that in the robot group （P<0.05）, and the combined group was significantly higher than the blood flow restriction group（P<0.05）. The average stride length and average stride frequency in the combined group and the robot group were significantly higher than those in the blood flow restriction group （both P<0.05）, and there was no difference between the two groups （both P>0.05）. The total length of the center of gravity movement trajectory in the combined group and the robot group was significantly shorter than that in the blood flow restriction group （P<0.05）, and the combined group was significantly shorter than the robot group （P<0.05）. The Lysholm score of the combined group was significantly higher than that of the flow restriction group and the robot group （P<0.05）, and there was no difference between the flow restriction group and the robot group （P>0.05）. Conclusions The lower limb rehabilitation robot combined with blood flow restriction training has a synergistic effect in the early rehabilitation of patients after anterior cruciate ligament reconstruction,which can significantly improve lower limb strength and gait function, as well as the overall function of knee joint.]]> Objective To evaluate the effects of preoperative high-intensity interval training （HIIT） on the rehabilitation effect of total knee arthroplasty（TKA）in patients with knee osteoarthritis （KOA）. Methods A total of 50 KOA patients scheduled for unilateral TKA at the Characteristics Medical Center of Chinese People’s Armed Police Force between January 2020 and June 2024 were enrolled and randomly assigned into an observation group or a control group, with 25 patients in each group. The observation group underwent a 6-week preoperative high-intensity interval training （HIIT）, while the control group received moderate-intensity training for 6 weeks. Assessments were conducted at baseline （T0）, preoperatively （T1）, and at 1 month （T2） and 3 months （T3） postoperatively, including maximal oxygen uptake （VO2max）, Hospital for Special Surgery （HSS） knee score, visual analog scale （VAS） for pain, passive range of motion （PROM）, 30-second sit-to-stand test （STS）, and wall-sit time （IST）. The incidence of adverse events during the intervention period was also compared between the two groups. Results Baseline features and outcome measures showed no significant differences between groups at T0. Compared with the control group,the observation group demonstrated significantly higher VO2max at all post-intervention time points （T1-T3） （P<0.05）. At T3, the observation group achieved superior outcomes in HSS score （87.84 ± 3.01）, PROM （114.00）, and VAS score 1.50 （P<0.05）, as well as in STS （22.54 ± 3.14） repetitions and IST （70.60 ± 14.27） s （P<0.05）. Generalized estimating equation analysis revealed that age was negatively correlated with VO?max （P<0.05）, and BMI had a significant adverse effect on PROM and IST （P<0.05）. No severe adverse events were reported, confirming the safety and feasibility of the HIIT protocol. Conclusions Preoperative HIIT is a safe and feasible preoperative exercise intervention method, which can improve the cardiopulmonary fitness,knee joint function,and lower limb muscular endurance in KOA patients,and alleviate postoperative pain.]]> Objective To review the existing clinical studies systematically and objectively evaluate the efficacy and safety of Kanglaite injection combined with chemotherapy in the treatment of gastric cancer, so as to provide useful evidence for clinic. Methods Through comprehensive retrieval of different Chinese or English databases, the time limit for all database literature retrieval is from the establishment of the database to October 2024. Two researchers independently conducted literature searches on the database, screened the literature according to inclusion and exclusion criteria, and evaluated the final eligible included literaure. The quality of the included literature was assessed using the Cochrane tool. The data analysis was conducted using the Review Manager 5.4 statistical software. Relative risk was used as the effect indicator for the outcome analysis of binary variable data, and mean difference was used as the effect indicator for the outcome analysis of quantitative data. The heterogeneity among different research results was compared using the I² test, and the fixed-effect model or random-effect model was selected for data analysis based on whether there was heterogeneity. Results A total of 15 eligible clinical studies were included, with 551 patients in the experimental group and 533 patients in the control group. Compared to the control group, Kanglaite Injection combined with chemotherapy demonstrated significant improvements in the complete remission rate （RR=1.60, 95% CI =1.24~2.06, P<0.01）, partial remission rate （RR=1.22, 95% CI =1.03~1.45, P=0.02）, disease efficiency rate （RR=1.34, 95% CI =1.19~1.50, P<0.01）, disease control rate （RR=1.19, 95% CI =1.11~1.28, P<0.01）, and quality of life improvement （RR=2.02, 95% CI =1.49~2.75, P<0.01）. Conversely, there were significant reductions in the disease progression rate （RR=0.56, 95% CI =0.43~0.72, P<0.01）, CEA （MD=-17.85, 95% CI =-26.54~-9.15,P<0.01）, CA199 （MD=-18.09, 95% CI =-35.08~-1.10, P<0.01）, CD4/CD8 ratio （MD=0.18, 95% CI =0.08~0.27,P<0.01）, and quality of life deterioration （RR=0.36, 95% CI =0.23~0.57, P<0.01）. However, there was no significant difference in the disease stabilization rate （RR=0.93, 95% CI =0.75~1.15, P=0.49）. In terms of adverse reactions, compared with the control group, the incidence of leukopenia, neurotoxicity, and gastrointestinal reactions significantly decreased after treatment with Kanglaite injection combined with chemotherapy for gastric cancer （P<0.05）. Conclusions Compared with patients treated with chemotherapy alone for gastric cancer, the combination of Kanglaite injection can improve clinical efficacy, improve quality of life, and reduce the incidence of adverse reactions, which can be further promoted in clinical practice.]]> Objective To analyze the condition of musculoskeletal injury(MSIs)in hospitalized soldiers of PAP,to put forward the corresponding prevention measures. Methods The medical records of MSIs inpatients of a certain PAP from 2021 to 2023 were collected. The distribution of military rank, classification criteria for injuries, year and disease type were analyzed. Results 353 case of MSIs were include in the evaluation. the proportion of officers, sergeants and conscripts was 19.26%, 52.98% and 27.76%. Joint injury (37.68%) and lower limb bone injury (33.43%) were the main injury types. Knee joint injury accounted for 92.23% of moderate trauma. Conclusion Sergeants are the main group of MSIs. The bones and joints of lower limbs are the most important parts of MSIs, and corresponding preventive measures should be taken for the key groups and parts.]]>