Objective To investigate the protective effect of quercetin against benzo(a)pyrene (BaP)-induced damage in human lung adenocarcinoma A549 cells by regulating Drp1 expression to improve mitophagy. Methods The A549 cells were randomly divided into 4 groups: a control group (normal culture), a BaP group (treated with BaP), a BaP+Quercetin group (quercetin intervention + BaP treatment), and a BaP+Mdivi-1 group (Drp1 inhibitor Mdivi-1 + BaP treatment). Cell viability was measured by CCK8 assay, apoptosis was observed by TUNEL staining, cell volume was observed by phalloidin staining, changes in organelles were examined by transmission electron microscopy, and the expression levels of autophagy-related proteins (Drp1, PINK1, Parkin) was analyzed by Western blotting. Results Compared with the Control group, the BaP group showed reduced cell viability (P<0.05), increased apoptosis rate and elevated expression of autophagy-related proteins (Drp1, PINK1, Parkin) increased (P<0.05). Compared with the BaP group, the cell viability in the BaP + Quercetin group and the BaP + Mdivi-1 group increased (P<0.05), and the apoptosis rate and the expression of autophagy-related proteins (Drp1, PINK1, Parkin) decreased (P<0.05). Conclusions Quercetin can regulate the expression of Drp1 to improve abnormal mitophagy and achieve the protective effect on A549 cells damage induced by BaP.
Key words
Drp1 /
mitophagy /
quercetin /
benzo(a)pyrene /
lung adenocarcinoma
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