Effect of DBC1 on biological behavior of liver cancer

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Medical Journal of the Chinese People Armed Police Forces ›› 2012, Vol. 23 ›› Issue (10) : 871-874.

GU Yong,DUAN Wei,MENG Hongtao,and WU Xiuhua. Department of Digestive Diseases,Shaanxi Provincial Crops Hospital of Chinese People’s Armed Police Forces,Xi’an⁷¹⁰⁰⁵⁴,China

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Abstract

Objective To study the effect of deleted genes of breast cancer 1(DBC1) onproliferation and apoptosis in liver cancer cells. Methods pcDNA3.1-DBC1 vector was constructed and transfected into human liver cancer cell line SMMC-7721. MTT assay and flow cytometry were performed to detect the effect of DBC1 on the proliferation, cycle, and apoptosis of cells. Results Compared with cells transfected with the control vector, entropic expression of DBC1 gene with pcDNA3.1-DBC1 vector significantly inhibited SMMC-7721 proliferation. Entropic expression of DBC1 caused a significant increase in the percentage of cells in the G1 phase （51.0% vs 64.9%, P=0.002），a significant decrease in the percentage of cells in the S phase （29.7% vs 14.0%, P

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GU Yong,DUAN Wei,MENG Hongtao,and WU Xiuhua. Department of Digestive Diseases,Shaanxi Provincial Crops Hospital of Chinese People’s Armed Police Forces,Xi’an,China. Effect of DBC1 on biological behavior of liver cancer[J]. Medical Journal of the Chinese People Armed Police Forces. 2012, 23(10): 871-874

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