Expression of IL-23 in nasal polyps and its significance

DING  Guo-Chen; WANG  Ya-Hui; SI   Ying; WANG   Yan; CAO  Zhi-Wei

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Medical Journal of the Chinese People Armed Police Forces ›› 2012, Vol. 23 ›› Issue (11) : 976-978.

Expression of IL-23 in nasal polyps and its significance

DING Guochen¹,WANG Yahui¹,SI Ying²,WANG Yan¹,and CAO Zhiwei³

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Abstract

Objective To investigate the expression and role of interleukin-23(IL-23) in nasal polyp patients. Methods NP tissues of 15 nasal polyp(NP) patients and 15 simple deviations of normal inferior turbinate tissues of nasal septum patients were collected. mRNA and protein expression of IL-23 were detected by means of immunohistochemistry and RT-PCR. Results Immunohistochemistry staining demonstrated that there were far more IL-23 positive cells in NP patients than in normal controls. The average expression level of IL-23 protein in NP tissues (0.321±0.023) was significantly different (P<0.01) from that of normal inferior turbinate tissues (0.199±0.011). RT-PCR demonstrated that the average expression level of IL-23p19mRNA in NP tissues (0.676±0.077) was significantly different (P＜0.01) from that in normal inferior turbinate tissues(0.212±0.015). Conclusions IL-23 may be involved in the chronic inflammation of NP.

Key words

interleukin-23 / nasal polyp / immunohistochemistry / RT-PCR

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DING Guochen,WANG Yahui,SI Ying,WANG Yan,and CAO Zhiwei. Expression of IL-23 in nasal polyps and its significance[J]. Medical Journal of the Chinese People Armed Police Forces. 2012, 23(11): 976-978

References

［1］姜晓丹,李琳,朱冬冬.2010年同一气道　同一疾病国际论坛纪要［J］.中华耳鼻咽喉头颈外科杂志,2010，45（12）:1049-1050.
［2］ IVANOV S,BOZINOVSKI S,BOSSIOS A,et al.Functional relevance of the IL-23/IL-17 axis in lnugs invivo ［J］.Am J Resoir Cell Mol Biol,2007,36(4):442-451.
［3］ KIKLY K,LIU L,NA S,et al.The IL-23/Th(17)axis:therapeutic targets for autoimmrne inflammation ［J］.Curr Opin Immunol,2006,18(6):670-675.
［4］ Chen Y S，Arab S F，Westhofen M，et al．Expression of intedeukin-5，interleukin-8,and interleukiu-10 mRNA in the ostecmeatal complex in nasal polypnsis［J］．Am J Bhinol，2005，19(2)：117-123．
［5］ Hamilos D L，Leung D Y，Huston D P，et al．GM-CSF，IL-5 and RANTES immunoreaetivity and mRNA expression in chronic hyperplastic sinusitis with nasal polyposis［J］．Clin Exp Allergy，1998，28(9)：1145-1152．
［6］ OPPMANN B,LESLEY R B ,BLOM J C,et al.Novel p19 protein engages IL-12p40 to form a cytokine,IL-23,with biological activities similar as well as distinct from IL-12［J］.Immunity,2000,13:715-725.
［7］ Li J,Gran B,Zhang G X,et al.Differential expression and regulation of IL-23 and IL-12 subunits and receptors in adult mouse microglia［J］.J Neurol Sci,2003,215(1-2):95-103.
［8］ Lubberts E,Koenders M I,Vanden W B.The role of T-cell interleukin-17 in comducting destructive arthritis:lessons from animal models ［J］.Arthritis Res Ther,2005,7(1):29-37.
［9］ Fuss I J,Becker C,Yang Z,et al.Both IL-12p70 and IL-23 are synthesizactive Crohn’s disease and are down-regulated by treatment with anti-IL-12p40 monoclonal antibody［J］.Inflamm Bowel Dis,2006,12(1):9-15.
［10］ Yuan X,Hu J,Belladona M L,et al.Interleukin-23-expressing bone matrowderived neural stem-like cells exhibit antitumor activity against intracranial glioma［J］.Cancer Res,2006,66(5):2630-2638.
［11］ Dewald G,Bork K.Missense mutations in the coagulation factor Ⅻ（Hageman factor） gene in
hereditary angioedema with mormal Cl inhibitor ［J］.Biochem Biophys Res Commun,2006,343(4):1286-1289
［12］ Bernstein I L.Hereditary angioedema:a current state-of –the-art review,Ⅱ：historical perspective of non-histamine-induced angioedema ［J］.Ann Allergy Asthma Immunol,2008,100(1 Suppl 2):S2-S6.
［13］ Wakashin H,Hirose K.Maezawa Y,et al.IL-23 and Thl7 cells enhance Th2-cell- mediated eosinophilic airway inflammation in mice［J］.Am J Respir Crit Care Med,2008,178:1023-1032．
［14］刘阳，刘争，崔永华，等.白细胞介素23在变应性鼻炎患者鼻黏膜中的表达及意义［J］.临床耳鼻咽喉头颈外科杂志,2010,24(14):638-640.