Objective To investigate the effects of resveratrol on proliferation of human bladder cancer cell line T24，and the role of miR-34a in its mechanism. Methods T24 cells were treated with resveratrol in different concentrations for 24 h. Cell proliferation was validated by MTT. Flow cytometry was used to analyse apoptosis. The expression of miR-34a was detect by real-time PCR. Results Resveratrol could inhibit the proliferation of T24 in a dose dependent manner, induce apoptosis and increase the expression of p53 significantly. Furthermore, the level of miR-34a in T24 cells was higher in resveratrol-treated group than that in the control group. Conclusions Resveratrol can suppress the proliferation of human bladder cancer cells, which may be attributed to inducing apoptosis, up-regulating miR-34a and p53.
Key words
resveratrol /
bladder cancer /
apoptosis /
miR-34a /
p53
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(2012-05-19
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