Objective To investigate the feasibility of autologous MSCs /Chondro-Gide engineered cartilage regeneration technology in cartilage regeneration therapy. Methods Twelve goats were randomly divided into experimental group, AMIC treatment group, and control group(n=8). The experimental group was treated by autologous MSCs / Chondro-Gide engineered technology; AMIC treatment group by AMIC technology, while the control group did not receive any implanted material. Gross, arthroscopic, radiological and histological observation and modified Wakitani score were used to evaluate the regeneration of articular cartilage defect repair eight weeks after operation. Results After eight weeks, cartilage defect repair was effective in experimental group. MRI and arthroscopy showed cartilage defects with smooth surface and good flexibility. Histology illustrated naive cartilage cells distributed across the repair surface. Histology of AMIC group and control group illustrated fibrous tissue covering the area of cartilage defect. On 8 and 16 weeks, Wakitani scores showed better repair effect in the experimental group than in AMIC treatment group and control group. Conclusions Autologous MSCs/Chondr-Gide engineered cartilage regeneration technology, with little trauma and evident user-friendliness, can significantly promote the regeneration repair of arthrosis cartilage defect; being of significant scientific value and clinical application.