Objective To investigate the distribution and drug resistance of isolates from blood specimens in this hospital from 2006 to 2011 so as to contribute to clinical diagnosis and treatment. Methods Isolates were obtained from blood culture bottles. The isolates were identified and the results of antibiotic resistance were analyzed by the  half-automated blood culture system.Results 305 strains were isolateded from 2300 blood specimens (13.3%)，including 172 Gram-negative （G-）bacteria (56.39%), 114 Gram-positive（G+）bacteria (37.38%), and 19 fungi(6.23%). Gram-negative bacteria were more susceptible to meropenem , piperacillin / tazobactam, levofloxacin, amikacin, and fosfomycin than to the cephalosporin antibiotics. Gram-positive bacteria were most susceptible to vancomycin. Conclusions The species and drug resistance of the bacteria isolated from blood specimens have changed. More attention should be paid to the detection and surveillance of bacterial resistance in blood culture to promote the rational use of antibiotics.