Objective To investigate the distribution and resistance of pathgenic bacteria in urinary tract infection and offer scientific basis for proper use of antibiotics.  Method Species identification and antibiotics susceptibility test were performed by Walkway 96 automicroscan systems(America).  Results A total of 246 strains of bacteria were isolated from  urine samples , most of which were Gram-negative bacteria with Escherichia coli as the most common. Enterococci were the most common Gram-positive bacteria, especially for E. faecalis and E. faecium. Fungal infection also increased. There was one case of vancomycin-resistant Enterococc (VRE) in invro antibiotics susceptibility test. The detection rate of ESBLs producing E. coli, Klebsiella penumoniae and Proteus was 51.4%, 47.1% and 16.7%, respectively. The susceptiblity rate of entrobacteriaceae to imipenem was 100.0%.  Conclusions It is  of great significance to analyze the distribution and antibiotic resistance of isolated bacteria in urine tract infection to control resistant strains.