Objective To establish an underwater blast injury model of the spinal cord for the study of the overpressure range of blast injury to the spinal cord and its wound characteristics. Methods Eighteen rats were divided into three groups according to the blast overpressure. All the rats underwent laminectomy at the T9-10 level. Then the rats were fixed in a capsule and only the exposed spinal cord was affected by shock wave. The explosive device containing 10 g RDX was used to produce blast waves underwater and the blast overpressure was measured at different distance between the explosive device and the exposed surface of the spinal cord. Then another 12 rats were divided into two groups. In the injury group, all the rats underwent standard blast injury to the spinal cord, and pathological changes of the spinal cord were observed. Results The overpressure of blast waves was 10, 5, 3MPa at 0.3, 0.8, 1.5 m, respectively, from the explosive devive. When the overpressure was 3MPa, the blast extended the MEP latent period by 1.0 ms or reduced the amplitude by 10%, while the rats were still alive. Pathological results of the spinal cord revealed a large scattered hemorrhagic necrosis area, especially in gray matter, and disorganized myelin sheath structure. Conclusions The present experiment demonstrates the overpressure range of blast injury to the spinal cord and provides an underwater blast injury model of the spinal cord. The model is simple, safe and effective.
Key words
spinal cord blast injury /
model
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References
［1］ Cooper G J, Maynard R L, Cross N L, et al . Casualties from terrorist bombings［J］. J Trauma,1983,23: 955–967.
［2］ Leibovici D, Gofrit O N, Stein M, et al . Blast injuries: bus versus open-air bombings-A coMParative study of injuries in survivors of open-air versus confined-space explosions［J］. J Trauma, 1996,41: 1030–1035.
［3］ Phillips Y Y. Primary blast injuries［J］. Ann Emerg Med,1986, 15: 1446-1450.
［4］ Jonsson A, Clemedson C J, Sundqvist A B. Dynamic factors influenceing the production of lung injury in rabbits subjected to blunt chest walliMPact［J］. Aviat Space Environ Med,1979,50(4):325-337.
［5］ 王昭领，刘彦普，雷德林，等. 点爆源爆炸伤模型建立及颌面部损伤特点［J］. 中国急救医学，2002,22（5）:253-254.
［6］ 陈 林, 王正国, 刘荫秋. 5.56 mm 和7.62 mm 弹压力波对培养大鼠肝脏细胞的作用［J］. 中国生物医学工程学报，1997，16 (1):18-22.
［7］ 〖ZK(#〗王建民，王正国，朱佩芳，等. 冲击波对肺血管内皮细胞通透性影响的机制［J］. 重庆大学学报,2002,25 (2):45-48.
［8］ 王正国，杨志焕，冷 光，等. 冲击伤的实验研究［J］. 中华医学杂志,1989，69(1):7-11.
［9］ Bauman R A, Ling G S, Tong L, et al . An introductory characterization of a combat-casualty-care relevant swine model of closed head injury resulting from exposure to explosive blas t ［J］. J Neurotrauma, 2009, 26: 841-860.
［10］ Wolman L. Blast injury of the spinal cord［J］. Paraplegia, 1967, 5:83-88.
［11］ Yuqing Wang, Zhengxu Ye, Xueyu Hu, et al . Morphological changes of the neural cells after blast injury of spinal cord and neuroprotective effects of sodium β-aescinate in rabbits［J］. Injury, 2010,41:801-810.
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