Objective To study the effect of bromocriptine on expression of Bax and bcl-2 in rat prolactinoma. Methods Firstly, to develop prolactinoma rats model. Adult Wistar rats were divided into two groups at random. The rats in control group were subscutaneously implanted with a blank implant . Rats in 17β-estradiol group were implanted with 17β-estradiol -containing implants. Secondly, Rats in 17β-estradiol group were divided into two groups at random, i.e. placebo group and bromocriptine group. Water was given to rats in placebo group. Bromocriptine was orally adminstered to rats in bromocriptine group. After 4 weeks of treatment, all the animals were sacrificed. Each pituitary gland was weighed. Serum prolactin(PRL) levels were measured by RIA. Expression level of Bax and bcl-2 in pituitary tissue were measured by Western blotting. Results (1)The weights of pituitary gland and PRL levels in 17β-estradiol group were significantly higher than those in control group (P <0.01,repectively). (2)The expression level of bcl-2 in bromocriptine group was significantly lower than that in placebo group (P<0.01). (3)The expression level of Bax in bromocriptine group was significantly higher than that in placebo group (P<0.01). Conclusion Bromocriptine has potential preventive effect against estrogen-induced prolactinoma in rats. The decrease of bcl-2 expression level and increase of Bax expression level may be involved in the mechanism of anti-prolactinoma effect of bromocriptine.
Key words
prolactinoma /
rats /
apoptosis /
Bax /
bcl-2
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