Objective To detect HBV-M and HBV-DND in the lac feminium of HBV-infected pureperas in order to evaluate the safety of breast feeding. Methods ELISA standing type and oscillator type brooding-methods were adopted to detect HBsAg in the lac feminium qualitivly while fluorescent quantitation PCR method was applied to detect HBV-DNA in the serum and lac feminium. Resluts The positive rate of HBV-DNA was 52.1%（75/144）in the serum of 144 HBV-infected puerperas and 34.7%（50/144）in the lac feminium. Moreover，66（45.8%）cases of positive HBsAg were detected by the standing type of brooding-method and 75（52.1%） by the oscillator type brooding-method. Conclusions HBsAg and HBV-DNA in the lac feminium should be detected simultaneously to improve the safety of breast feeding. Oscillator type brooding-method should be adopted to detect lac feminium to enhance the positive rate of HBsAg in laboratories unable to detect HBV-DNA.