Objective To study the clinical features and pathology differences between the H.pylori-positve and the H.pylori- negative peptic ulcers. Methods 112 patients were enrolled in H.pylori-positve group and 86 in H.pylori-negative group. The clinical, endoscopic and pathological characteristics of these two groups were compared. Results There ware no significant differences in demographic characteristics and concomitant diseases between the two groups.The patients in H.pylori-positve group had higher rate of taking NSAIDs and the gastrointestinal symptom score,more abdominal pain but less nausea and vomiting(P＜0.05). In the two groups of patients, DU had major proportion. The GU proportion was higher in the H.pylori-negative group, but in the positive group DU proportion was higher (P＜0.05). Under endoscopy, patients in H.pylori-negative group had more gastric ulcer but less duodenal ulcer, greater peptic ulcer diameter and higher tendency to cause bleed(P＜0.05).No significant differences were found between the H.pylori-positve and the H.pylori- negative patients in incidence of complex ulcer,ulcer stage, number of ulcers and bile reflux. H.pylori-negative patients’ degree and activity of mucosal inflammation, lymphoid tissue hyperplasia and intestinal metaplasia were more than those in H.pylori- negative patients(P＜0.05), The degree and activity of mucosal inflammation were aggravated with increasing H.pylori density(P＜0.05). Conclusions H.pylori-positve peptic ulcers have higher gastrointestinal symptom score,more DU,less size and more pathological changes,while the H.pylori- negative peptic ulcers have a close relationship with NSAIDs,more GU,larger size and a higher tendency to bleed.