Alpha-crystallin promotes OECs survival in vitro

WANG Yanhua; WANG Dongwu; and YIN Zhengqin.

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Medical Journal of the Chinese People Armed Police Forces ›› 2013, Vol. 24 ›› Issue (4) : 316-318.

Alpha-crystallin promotes OECs survival in vitro

WANG Yanhua^¹,²,WANG Dongwu^²,and YIN Zhengqin^¹.

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Abstract

Objective To investigate the effect of α-crystallin on the survival and proliferation of olfactory ensheating cells(OECs) in vitro. Methods OECs were cultured and treated with α-crystallin in DMEM/F12 or DMEM/F12 only. The number of surviving OECs was counte for after cultured 5days. The number of Brdu-labeled OECs was counted after Brdu was added in DMEM/F12. And cell cycle was analyzed by flow cytometry. Results α-crystallin promoted the survival of OECs at 6-13 days. The Brdu-labeled OECs in α-crystallin group was more in number than in control group. And the cell percentage of G₂/M state and S state was higher in α-crystallin group than in control group. Conclusions α-crystallin promotes OECs survival and proliferation.

Key words

α-crystallin / olfactory ensheathing cells / proliferation

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WANG Yanhua,WANG Dongwu,and YIN Zhengqin.. Alpha-crystallin promotes OECs survival in vitro[J]. Medical Journal of the Chinese People Armed Police Forces. 2013, 24(4): 316-318

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