Serum microRNA-122 as a specific marker of sepsis diagnosis

DENG Jie; WANG Huijuan; SU Longxiang; ZHANG Xin; XIAO Kun; JIA Yanhong; and XIE Lixin

Medical Journal of the Chinese People Armed Police Forces ›› 2013, Vol. 24 ›› Issue (5) : 383-386.

DENG Jie^¹,WANG Huijuan^²,SU Longxiang^²,ZHANG Xin^¹,XIAO Kun^¹,JIA Yanhong^¹,and XIE Lixin^¹

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Abstract

Objective To study the dynamic changes in serum miRNA-122 levels during sepsis and the correlation between serum miRNA-122 and sepsis severity. Methods Fifty-two sepsis patients (including 23 non-severe septic patients and 29 severe septic patients)and 23 healthy controls were recruited in the study. The expression levels of serum miRNA-122 were quantified by quantitative reverse transcriptase polymerase chain reaction assays (qRT-PCR).Levels of serum miRNA-122 were detected on the 1 st, 3 rd, 5 th, 7 th, 10 th and 14 th day after their admissions in intensive care unit. All the patients clinical information was also recorded. Results After comparison, levels of serum miRNA-122 in sepsis patients on every observation time point were all significantly lower than those in healthy controls (P＜0.01). The receiver operating characteristic curve was obtained and the area under curve was calculated on the levels of serum miRNA-122 on the first day of their admissions. When the cut-off point was set at 0.710, miRNA-122 had a specificity of 95.7% and a sensitivity of 53.8%. The expression levels of serum miRNA-122 in severe sepsis group were significantly higher than in sepsis group on each observation time point (P＜0.01). Conclusions Serum miRNA-122 can used as a specific biomarker for diagnosis of sepsis. And miRNA-122 might be correlated with the organ injuries of septic patients.

Key words

sepsis / microRNA-122 / microRNA / diagnosis

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DENG Jie,WANG Huijuan,SU Longxiang,ZHANG Xin,XIAO Kun,JIA Yanhong,and XIE Lixin. Serum microRNA-122 as a specific marker of sepsis diagnosis[J]. Medical Journal of the Chinese People Armed Police Forces. 2013, 24(5): 383-386

References

[1] Levy M M, Fink M P, Marshall J C, et al. Ramsay G: 2001 SCCM/ESICM/ACCP/ATS/SIS International Sepsis Definitions Conference[J]. Crit Care Med, 2003, 31(4):1250-1256.

[2] Wang H, Zhang P, Chen W, et al. Evidence for serum miR-15a and miR-16 levels as biomarkers that distinguish sepsis from systemic inflammatory response syndrome in human subjects[J]. Clin Chem Lab Med, 2012, 50(8): 1423-1428.

[3] Wang H, Meng K, Chen W J, et al. Serum miR-574-5p: a prognostic predictor of sepsis patients [J]. Shock, 2012, 37(3): 263-267.

[4] Bone R C, Balk R A, Cerra F B, et al. Definitions for sepsis and organ failure and guidelines for the use of innovative therapies in sepsis. The ACCP/SCCM Consensus Conference Committee. American College of Chest Physicians/Society of Critical Care Medicine[J]. Chest ,1992, 101(6):1644-1655.

[5] Ambros V. The functions of animal microRNAs [J]. Nature, 2004, 431: 350–355.

[6] Vasilescu C, Rossi S, Shimizu M, et al. MicroRNA fingerprints identify miR-150 as a plasma prognostic marker in patients with sepsis [J]. PLoS One, 2009, 4(10): e7405.

[7] Wang Jiafeng, Yu Manli, Yu Guang, et al. Serum miR-146a and miR-223 as potential new biomarkers for sepsis[J]. Biochem Biophys Res Commun, 2010, 394(1): 184-188.

[8] Wang Huijuan, Zhang Pengjun, Chen Weijun, et al. Serum MicroRNA Signatures Identified by Solexa Sequencing Predict Sepsis Patients’ Mortality: A Prospective Observational Study [J]. PLoS One, 2012, 7(6): e38885.

[9] Hotchkiss R S, Nicholson D W. Apoptosis and caspases regulate death and inflammation in sepsis?[J]. Nat Rev Immunol, 2006, 6: 813-822.

[10] Hotchkiss R S, Tinsley K W, Swanson P E, et al. Sepsis-induced apoptosis causes progressive profound depletion of B and CD4+ T lymphocytes in humans[J]. Immunol,2001,166: 6952–6963.

[11] Hotchkiss R S, Tinsley K W, Swanson P E, et al. Depletion of dendritic cells, but not macrophages, in patients with sepsis[J]. Immunol, 2002,168: 2493–2500.

[12] Jia Y X, Pan C S, Yang J H, et al. Altered L-arginine/nitric oxide synthase/nitric oxide pathway in the vascular adventitia of rats with sepsis[J]. Clin Exp Pharmacol Physiol, 2006, 33(12):1202-1208.

[13] Chang J, Nicolas E, Marks D,et al. miR-122, a mammalian liver-specific microRNA, is processed from her mRNA and may downregulate the high affinity cationic amino acid transporter CAT-1 [J]. RNA Biol, 2004, 1(2): 106-113.