Objective To investigate the anti-proliferative effect and underlying mechanisms of the combination of curcumin and adriamycin on breast cancer MCF-7 and MDA-MB-231 cells. Methods MTT was used to detect the cell viablility of MCF-7 and MDA-MB-231 cells after different concentrations of curcumin (Cur) and adriamycin(ADM) treatment. Calcusyn software was used for evaluating the combination index (CI). The proteins of PARP, Bcl-2, Bax, and NF-κB (p65) were detected by Western blotting. Results The combination of Cur and ADM synergisticly inhibited the growth of MCF-7 and MDA-MB-231 cells, with the ED50CI of 0.70 and 0.62, respectively. The Western blotting data showed that the combination of Cur and ADM could enhance the cleavage of PARP, and decrease the expression of Bcl-2, but had no affect on the expression of Bax in MCF-7 cells. In addition, we found that the combination treatment could inhibit the activation of NF-κB induced by ADM. Conclusions Cur sensitizes breast cancer to ADM in vitro most likely via inhibiting the activation of NF-κB signaling induced by ADM and subsequent increasing the apoptosis.
Key words
apoptosis /
curcumin /
adriamycin /
NF-κB
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