Quantitative analysis of p63 expression in lung cancers and its relationship with cancer cellular apoptosis

PDF(540 KB)

Medical Journal of the Chinese People Armed Police Forces ›› 2014, Vol. 25 ›› Issue (10) : 1029-1031.

ORIGINAL ARTICLES

Quantitative analysis of p63 expression in lung cancers and its relationship with cancer cellular apoptosis

WANG Lu¹ SHEN Hong²

Author information +

History +

Abstract

Objective To analyze the expression of protein p63 in normal lung and lung cancers quantitatively and judge its relationship with cellular apoptosis. Methods Lung cancer and normal lung tissues were collected and tissue arrays were prepared. The p63 expression was determined by immunohistochemistry and analyzed quantitatively. The cellular apoptosis was analyzed quantitatively by terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL). The final qualified results (positive unit, PU) were analyzed statistically. Results The PU values of the intensity of p63 protein expression in normal lung tissue, adenocarcinoma, large cell carcinoma and small cell carcinoma were all lower than that in squamous carcinoma (P＜0.01); The PU value of normal lung tissue was higher than those in adenocarcinoma, large cell carcinoma and small cell carcinoma (P＜0.01); while there was a positive correlation between P63 protein expression and apoptosis in all kinds of lung cancer (P＜0.01). Conclusions The extents of protein p63 expression are distinct on the normal lung and lung carcinoma tissues. The p63 expression and cellular apoptosis on the normal lung tissues are separated. However, p63 expression and cellular apoptosis on the lung malignant tissues are positively correlated.

Key words

p63 / lung cancer / apoptosis / quantitative analysis

Cite this article

EndNote

Ris (Procite)

Bibtex

Download Citations

WANG Lu SHEN Hong. Quantitative analysis of p63 expression in lung cancers and its relationship with cancer cellular apoptosis[J]. Medical Journal of the Chinese People Armed Police Forces. 2014, 25(10): 1029-1031

References

[1] Joerger A C, Rajagopalan S, Natan E, et al. Structural evolution of P53, P63, and p73: implication for heterotetramer formation[J]. Proc Natl Acad Sci U S A, 2009, 106(42):17705-17710.
[2] Thomason H A, Zhou H, Kouwenhoven E N, et al.Cooperation between the transcription factors P63 and IRF6 is essential to prevent cleft palate in mice[J].J Clin Invest, 2010,120(5):1561-1569.
[3] Huang W,Liu K.P53 family proteins provide new insights into lung carcinogenesis and clinical treatment [J].Zhongguo Fei Ai Za Zhi, 2013,16(8):422-426.
[4] Kim D H, Kwon M S. Role of fine needle aspiration cytology, cell block preparation and CD63, P63 and CD56 immunostaining in classifying the specific tumor type of the lung[J].Acta Cytol, 2010, 54(1):55-59.
[5] Shimada Y, Ishii G, Nagai K, et al. Expression of podoplanin, CD44, and P63 in squamous cell carcinoma of the lung[J]. Cancer Sci, 2009,100(11):2054-2059.
[6] NapsinA.TTF-1、CK5/6、CK7、P63在非小细胞肺癌支气管镜活检标本中的表达及意义[J].肿瘤防治研究,2013,40(1):76-78.
[7] Yao J Y,Chen J K. Roles of P63 in epidermal development and tumorigenesis[J]. Biomed J,2012,35(6):457-463.
[8] Glickman J N, Yang A, Shahsafaei A, et al.Expression of P53-related protein P63 in the gastrointestinal tract and in esophageal metaplastic and neoplastic disorders[J]. Hum Pathol, 2001,32(11):1157-65.
[9] Massion P P, Taflan P M, Jamshedur S M, et al. Significance of P63 amplification and overexpression in lung cancer development and prognosis[J].Cancer Res, 2003, 63(21):7113-7121.
[10] 梁喜林,孟晓,赵红.前列腺良恶性病变中P504S、P63、CK34βE12表达的研究[J].武警医学,2010,21(3):200-201.
[11] 刘换新,王彩霞,刘梅.宫颈毛玻璃细胞癌临床病理观察[J].武警医学,2013,24(2):139-142.
[12] 万蔚平, 赵妍妍, 吴开松.P63和CK5/6在肺癌组织中的表达及临床意义[J].中华临床医师杂志, 2014,8(3):444-447.
[13] Jacobs J J, Lehe C , Cammans K D, et al. An automated method for the quantification of immunostained human Langerhans cells[J]. J Immunol Method, 2001,247(1-2):73.
[14] Huang F,Shen H.Inter random number generator-based true random number generator[J].Di Yi Jun Yi Da Xue Xue Bao,2004,24(9):1091-1095.
[15] 韩伟.TGF-β₁和PTEN在非小细胞肺癌组织中的表达及相关关系定量分析[J].中国体视学与图像分析,2014,19(1):63-69.
[16] Pavlovic V, Sarac M. The role of ascorbic acid and monosodium glutamate in thymocyte apoptosis[J].Bratisl Lek Listy, 2010,111(6):357-360.