Effect of hyperbaric oxygenation treatment on expression of NF-κB p50 in the rat brain tissue after traumatic brain injury

YANG Bolin; WANG Huan; PENG Jun; XU Qiming; and YU Chao

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Medical Journal of the Chinese People Armed Police Forces ›› 2014, Vol. 25 ›› Issue (11) : 1131-1134.

Effect of hyperbaric oxygenation treatment on expression of NF-κB p50 in the rat brain tissue after traumatic brain injury

YANG Bolin, WANG Huan, PENG Jun, XU Qiming, and YU Chao

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Abstract

Objective To observe the effect of hyperbaric oxygenation treatment(HBOT) on expression of NF-κB(p50) in the rat brain tissue after traumatic brain injury(TBI) and discuss its mechanism. Methods Thirty-five Sprague-Dawley rats were divided into sham operation control group(5 rats), TBI group(15 rats) and HBOT group(15 rats). Based on the Feeney’s model of modified Allen’s method, experimental animals were treated with HBOT. The animals were sacrificed at the 8th hour, and on days 1,3,5,8,respectively. The expression of protein NF-κB was determined by Westernblotting. Results The expression of NF-κB was significantly higher in traumatic group than that in control group at the time of 8 hour and 1,3,5and 8 days after injury (P＜0.05).The level of NF-κB reached peak on the 3th day, and maintained a high status from 5 to 8 days. Compared with traumatic group, the NF-κB level was significantly lower in the HBOT group (P＜0.05). Conclusion HBOT can weaken NF-κB expression in traumatic brain tissue and inhibit effectively inflammatory response, which provides theoretical basis for treating severe traumatic brain injury.

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severe brain injury / hyperbaric oxygenation / rats / NF-κB

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YANG Bolin, WANG Huan, PENG Jun, XU Qiming, and YU Chao. Effect of hyperbaric oxygenation treatment on expression of NF-κB p50 in the rat brain tissue after traumatic brain injury[J]. Medical Journal of the Chinese People Armed Police Forces. 2014, 25(11): 1131-1134

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