Comparison of four commercially available assays for antibody to hepatitis B virus surface antigen

ZHANG Xiaoli; WANG Ruiling; SONG Na; WANG Wenjing; ZHANG Cui; ZHANG Xuedong; WANG Chongchong; and YANG Xiaoli

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Medical Journal of the Chinese People Armed Police Forces ›› 2014, Vol. 25 ›› Issue (12) : 1209-1211.

ORIGINAL ARTICLES

Comparison of four commercially available assays for antibody to hepatitis B virus surface antigen

ZHANG Xiaoli¹,WANG Ruiling¹,SONG Na¹, WANG Wenjing¹, ZHANG Cui¹,ZHANG Xuedong², WANG Chongchong²,and YANG Xiaoli¹

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Abstract

Objective To study four commercially available assays for antibody to hepatitis B virus surface antigen. Methods A total of 252 specimens were tested by Abbott i2000、Roche e601、Autobio AutoLumoA2000 and a ELISA kit, respectively,and the quantitative results and the performance characteristics of the different test systems were compared. Results The sensitivity and specificity of different manufacturer kits for Anti-HBs were beyond 93%. The detection rates of compared CLIA(Chemiluminescence Immunoassay) commercial Anti-HBs reagents had no significant difference (P>0.05). Conclusions The four commercial Anti-HBs reagents have false positive and false negative results; the correlation analysis for quantitative results is beyond 0.85.

Key words

antibody to hepatitis B surface antigen / gold standard / sensitivity / specificity / correlation analysis

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ZHANG Xiaoli,WANG Ruiling,SONG Na, WANG Wenjing, ZHANG Cui,ZHANG Xuedong, WANG Chongchong,and YANG Xiaoli. Comparison of four commercially available assays for antibody to hepatitis B virus surface antigen[J]. Medical Journal of the Chinese People Armed Police Forces. 2014, 25(12): 1209-1211

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