Objective To study the stability of the laser in situ keratomileusis (LASIK) flap in a rabbit model when subjected to vertical acceleration at ten times the force of gravity (+7Gz). Methods It was an experimental study. 24 eyes(12 Japanese white rabbits) with one week postoperative LASIK were randomly divided into 2 groups: the control group without any simulator; the vertical accelerated group which underwent a controlled rapid-sequence ejection at +7 Gz. in a cockpit ejection seat simulator. The stability of corneal flap was observed with optical microscope and transmission electron microscope (TEM). The stability of corneal flap was compared between the groups. Results The images under optical microscope and TEM showed that the interface tissue healing between the flap and stromal bed was clear without separation, and surrounded by regular collagen fibers in vertical accelerated group. The differences between the two groups was insignificant(P=0.64).Conclusions When subjected to a rapid vertical ejection at ten times the force of gravity, corneal flaps after LASIK in rabbit model are stable.