Objective To study the changes in mitochondrial bioenergetics, mitochondrial DNA (mtDNA) and oxidative damage in leukocytes during acclimatization to highland hypoxia. Methods Twenty-seven lowlanders quickly entered plateau. Leukocyte mitochondrial membrane potential, 8-oxodG in mtDNA, mtDNA copies and PGC-1α protein expression were measured in plain and plateau at 3 d,7 d,90 d after entry. Results As compared with plain group, membrane potential was significantly decreased (P<0.05); 8-oxodG amounts, mtDNA copies and PGC-1α expression were markedly increased at 3 d and 7 d (P<0.05). And at 90 d, mtDNA copies and PGC-1α expression were significantly decreased (P<0.05). As compared with 7 d group, membrane potential was significantly increased (P<0.05); 8-oxodG amounts, mtDNA copies and PGC-1α expression were markedly decreased at 90 d (P<0.05). Conclusions In the early phase of acclimatization to highland, leukocyte bioenergetics remodeling mainly depends on increased mitochondrial content. In the later phase of acclimatization to highland, leukocyte bioenergetics remodeling mainly depends on elevated level of single mitochondrial health and energy metabolism.
Key words
acclimatization to high-altitude /
leukocytes /
mitochondrial DNA /
copy number /
oxidative stress
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