sHLA-G expression levels in maternal serum and amniotic fluid and its correlations with premature rupture of membranes

XU  Jing-Jie; HANG  Mu-Lan; ZHAO  Wei

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Medical Journal of the Chinese People Armed Police Forces ›› 2014, Vol. 25 ›› Issue (8) : 812-815.

ORIGINAL ARTICLES

sHLA-G expression levels in maternal serum and amniotic fluid and its correlations with premature rupture of membranes

XU Jingjie,HANG Mulan,ZHAO Wei

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Abstract

Objective To study the roles of soluble human leukocyte antigen-G(sHLA-G)in the occurrence and development of premature rupture of membranes and their correlations by detecting premature rupture of memberes(PROM)and the levels of sHLA-G in maternal serum and amniotic fluid under normal pregnancy, and to evaluate the correlations of sHLA-G and IL-18 in predicting histological chorioamnionitis. Methods The levels of sHLA-G in maternal serum and amniiotic fluid under normal pregnancy were detected, which among 30 cases of pregnant women in the term premature rupture of membrances (TPROM) group, 30 cases in the preterm premature rupture of membranes (PPROM) group and 24 cases in the control group by enzyme linked immunosorbent assay. And its correlation with histological chorioamnionitis was discussed. The data were analyzed by using statistical software of SPSS13.0. Results (1)The levels of sHLA-G in maternal serum and amniotic fluid of PROM group were both higher than those in normal control group.The postpartum fetal membrane pathological examination in PROM group showed that the levels of sHLA-G in chorioamnionitis patients were higher than those in non-chorioamnionitis patients. and the differences were statistically significant(P<0.05).(2)For the relationship between the fetal membranes rupture time and the incidence of fetal membrane chorioamnionitis: the <8 h incidence rate was 7.14%,the 8-12 h incidence rate was 33.3%, the >12 h incidence rate was 64.2%; by comparing the incidences in three groups, the differences were statistically significant(P<0.05).It was considered that the incidence of infection increased with increasing rupture time. Conclusion The increase in the levels of sHLA-G in maternal serum andamniotic fluid might be involved in the incidence of premature rupture of membranes,or with chorioamnionitis. sHLA-G is speculated to be as one of the clinical diagnostic indexes of premature rupture of membranes and amniotic infection.

Key words

promature rupture of members / histological chorioamnionitis / soluble human leukocyte antigen-G

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XU Jingjie,HANG Mulan,ZHAO Wei. sHLA-G expression levels in maternal serum and amniotic fluid and its correlations with premature rupture of membranes[J]. Medical Journal of the Chinese People Armed Police Forces. 2014, 25(8): 812-815

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