Objective To analyze the expression levels of serum microRNA-122 (miR-122) in non-alcoholic fatty liver disease (NAFLD) patients, and its clinical significance. Methods Patients with normal liver, hyperlipidemia, non-alcoholic simply fatty liver(NAFL), non-alcoholic steatohepatitis(NASH)were included in this study. Blood samples of the patients were collected for analysis. Real time quantitative PCR technique was used to measure the expression level of serum miR-122. Results The expression levels of serum miR-122 was 5.94±1.21(2-△△Ct), 5.99±1.03(2-△△Ct), 8.67±0.72(2-△△Ct)and 10.55±0.83(2-△△Ct), respectively. The difference in the expression level between normal liver and hyperlipidemia patients was not statistically significant. Comparing with normal liver and hyperlipidemia patients, miR-122 presented higher expressions in NAFL patients(P<0.05), especially in NASH patients. Conclusion miR-122 plays an important role in NAFLD development and has early diagnostic value for indicating NAFLD. It may also be a potential therapeutic target in NAFLD with significant clinical value.
Key words
NAFLD /
fatty acid metabolism /
early diagnosis /
miR-122
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