Evaluation of TYMS gene expression level and its clinical significance in advanced lung adenocarcinoma

ZHANG Zhihao; ZHU Youcai; WU Dongqiang; XIAO Huaiqing; GUO Dawei; WANG Xiyong; CHEN Huafei; LI Xiaofeng; DU Kaiqi

Medical Journal of the Chinese People Armed Police Forces ›› 2015, Vol. 26 ›› Issue (1) : 19-22.

ORIGINAL ARTICLES

Evaluation of TYMS gene expression level and its clinical significance in advanced lung adenocarcinoma

ZHANG Zhihao, ZHU Youcai, WU Dongqiang, XIAO Huaiqing, GUO Dawei, WANG Xiyong, CHEN Huafei, LI Xiaofeng, DU Kaiqi

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Abstract

Objective To examine the relative expression of TYMS gene in 150 FFPE samples of advanced lung adenocarcinoma patients and to validate the method for differentiating TYMS gene expression levels in term of clinical significance. Methods Real time quantitative PCR and 2^-△Ct method was used to detect the expression of TYMS and internal reference gene. The median value was used as the cut-off value and retrospective validation was performed including the short and long term therapeutic response towards pemetrexed based chemotherapy. Results In the 150 samples we examined, the detection rate was 89.3% when both the TYMS and the internal reference gene were successfully amplified. TYMS expression was significantly correlated with late stage adenocarcinoma patients’ age groups (P=0.025) but not significantly correlated with patients’ gender, histology, stage or smoking history (P＞0.05). The ORR (Objective response rate) in TYMS high and low expression groups was 9.8% and 35.4%, respectively (P<0.05). The median progression free survival time in TYMS high and low expression group was 2.1 and 4.9 months, respectively (P=0.047). The median overall survival time in TYMS high and low expression group was 9.1 and 16.7 months, respectively (P=0.024). Conclusions The method for differentiating TYMS gene expression levels in the present study is suitable in late stage adenocarcinoma patients and sheds light on the standardization of TYMS expression detection. This method can also be used to predict the therapeutic efficiency of pemetrexed chemotherapy.

Key words

lung adenocarcinoma / TYMS / expression / cut-off value

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ZHANG Zhihao, ZHU Youcai, WU Dongqiang, XIAO Huaiqing, GUO Dawei, WANG Xiyong, CHEN Huafei, LI Xiaofeng, DU Kaiqi. Evaluation of TYMS gene expression level and its clinical significance in advanced lung adenocarcinoma[J]. Medical Journal of the Chinese People Armed Police Forces. 2015, 26(1): 19-22

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