Drug-release kinetics characterization of gentamicin from TiO2 nanotubes and its antibacterial activity in vitro

FENG Mingguang; LIU Zhongtang; WANG Liqiang; XU Li; YANG Haitao; WANG Jian; WANG Haiyang; and QING Shixin

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Medical Journal of the Chinese People Armed Police Forces ›› 2015, Vol. 26 ›› Issue (11) : 1129-1133.

ORIGINAL ARTICLES

Drug-release kinetics characterization of gentamicin from TiO₂ nanotubes and its antibacterial activity in vitro

FENG Mingguang¹,LIU Zhongtang², WANG Liqiang³, XU Li¹, YANG Haitao¹, WANG Jian¹, WANG Haiyang¹,and QING Shixin¹

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Abstract

Objective To determine the release kinetics of gentamicin from the TiO₂ nanotubes(TNS-G), and to detect its antibacterial activity in vitro. Methods TiO₂ nanotubes (NTS) were fabricated on the titanium surface by electrochemical anodization. These nanotubes were loaded with gentamicin using a lyophilization method and vacuum drying, and its pharmacokinetics was detected in phosphate buffer. Staphylococcus aureus was used to study the antibacterial properties of the NTS-G. There were three study groups: the commercially pure titanium(Cp-Ti)group, the NTS group, and the NTS-G group.We compared the antibacterial efficacy with each other by bacterial adhesion and colony counting in bacterial culture. Results Drug release of NTS-G could be divided into two parts: initial burst release and relatively slow release.The fast initial concentration was 51.50 μg/ml , and 73.13 μg/ml at the 6 th hours. Most of load gentamicin was released within approximately 9 hours.In the second phase, different drug release kinetics were observed, with very slow and linearly increasing cumulative release over a period of 90 h. The drug concentration was maintained at about 89.10 μg/ml. We found that NTS-G could significantly inhibit bacterial adhesion and bioflm formation, compared with Cp-Ti or NTS, (P<0.05). Conclusions Titanium dioxide nanotubes loaded with antibiotics can deliver a high concentration of antibiotics locally at a specifc site, thereby providing a new strategy to prevent implant-associated infections.

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TiO₂ nanotubes / gentamicin / Drug-release kinetics / Staphylococcus epidermidis / antibacterial activity

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FENG Mingguang,LIU Zhongtang, WANG Liqiang, XU Li, YANG Haitao, WANG Jian, WANG Haiyang,and QING Shixin. Drug-release kinetics characterization of gentamicin from TiO₂ nanotubes and its antibacterial activity in vitro[J]. Medical Journal of the Chinese People Armed Police Forces. 2015, 26(11): 1129-1133

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