Objective To explore the contribution of EGB761 to the proliferation and apoptosis of Ewing’s sarcoma VH-64 cell in vitro. Methods CCK-8 assay was used to measure 50,100 and 200 mg/L which its effects on the proliferation of Ewing’s sarcoma VH-64 cell cultured with different concentrations of allicin for 12 h, 24 h, 48 h, AnnexinV-FITC and PI assay by flow cytometer (FCM) were applied to detect the apoptosis induced by EGB761. The changes of cell cycle were detected by FCM. Results EGB761 suppressed the proliferation of Ewing’s sarcoma VH-64 cell in a concentration-and time-dependent manner EGB761 induced cellular apoptosis of the Ewing’s sarcoma VH-64 cell in a concentration-dependent manner. With EGB761 100, 200 mg/L intervention Ewing’s sarcoma VH-64 cell, flow cytometer (FCM) test showed that the cell apoptosis rate were 6.85%±0.36% and 14.36%±0.23%,which were significant higher than that of control group (P< 0.01). Conclusions EGB761 significantly suppresses the growth of Ewing’s sarcoma VH-64 cell, induces the apoptosis of Ewing’s sarcoma VH-64 cell. Therefore resveratrol may be considered as a possible treatment medicine for Ewing’s sarcoma.
Key words
EGB761 /
VH-64 cell /
Ewing’s sarcoma /
apoptosis
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