Protective effect and mechanism of amifostine on intestinal acute radiation sickness

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Medical Journal of the Chinese People Armed Police Forces ›› 2015, Vol. 26 ›› Issue (8) : 797-800.

ORIGINAL ARTICLES

Protective effect and mechanism of amifostine on intestinal acute radiation sickness

ZHAO Zhiwei^1,2, CUI Yu²,WANG Limei², SHAN Yajun²,TANG Hongwei³, CONG Yuwen²

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Abstract

Objective To study the radioprotection effect and mechanism of amifostine (WR2721) on intestinal acute radiation sickness. Methods Based on the intestinal acute radiation sickness model, C57BL/6J mice were randomly divided into control group and group WR2721. Bone marrow cells from healthy mice were transplanted after 15 Gy ⁶⁰Co-γ shielded body irradiation. Then the survival rate in 30 d after irradiation, and the number of proliferative crypt cells and the small intestine mucosa situation with micro-cloning survival assay of mouse crypt cells at 3.5d post-irradiation were observed . Results The mice in control group died within 10 days, survival rate in WR2721 administrered group increased to 80%; in the control group and WR2721 drug group, the average number of crypts were 6.1±0.7, 28±1.1, the difference was statistically significant (P<0.0001); in control group and WR2721 administered group, villus heights were(51.48±2.04)μm, (73.29±3.24)μm, the difference was statistically significant (P<0.0001). Conclusions WR2721 has significantly radioprotective effect on intestinal acute radiation sickness by improving the survival rate of mice, promoting crypt cells regeneration and protecting the intestinal mucosa.

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WR2721 / radioprotection / intestinal acute radiation sickness / mechanism

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ZHAO Zhiwei, CUI Yu,WANG Limei, SHAN Yajun,TANG Hongwei, CONG Yuwen. Protective effect and mechanism of amifostine on intestinal acute radiation sickness[J]. Medical Journal of the Chinese People Armed Police Forces. 2015, 26(8): 797-800

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