Objective To repair bone defect in implant region using bone marrow mesenchymal stem cells-based tissue-engineering bone and investigate its osteogenesis potential. Methods Bilateral implantaion,two implants every side, was performed in the mandible premolar region of 3 Beagles and bone defects were created in the distal of every implant.Two bone defects in one side of mandible premolar region were randomly repaired with two types of grafts: PRP (n=6, the control group);and compound osteogenic-induced BMSCs with PRP(n=6, the experimental group). 1 and 3 months after transplation, gross investigation,X-ray examination and histological observation were conducted to examine bone formation. Results One month after implantation,the osteogenesis in experimental group was better than in the control group. We detected more bone matrix and less collagenous fibers in the experimental group.The bone density in the experimental group was higher than in the control group and the difference was significantly different[(101±8.99)vs(93±9.81),P≤0.05].Three months after implantation,the bone density in the experimental group tended to be higher than in the control group; however,the difference was not statistically significant[(100.75±12.05)vs(105±11.58)].But we detected more Haversian system in the experimental group. Conclusion Bone marrow stem cell-based tissue-engineered bone has high osteogenic potential around implants.
Key words
bone marrow mesenchymal stem cells /
tissue-engineered bones /
oral implantology
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