Effect of intermittent hypoxia on transverse aortic constriction of intestinal flora in a murine model

ZHOU Xianjie; LI Jun; LU Feixiang; XIA Jingyuan; and LIU Qingchun

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Medical Journal of the Chinese People Armed Police Forces ›› 2016, Vol. 27 ›› Issue (11) : 1121-1125.

ORIGINAL ARTICLES

Effect of intermittent hypoxia on transverse aortic constriction of intestinal flora in a murine model

ZHOU Xianjie¹,LI Jun², LU Feixiang¹, XIA Jingyuan¹, and LIU Qingchun¹

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Abstract

Objective To study the influence of intermittent hypoxia on transverse aortic constriction (TAC) of intestinal flora in a murine model.Methods Eighteen C57BL/6 mice were evenly and randomly divided into three groups: Group C, Group TC and Group TH. Twelve of these mice were subjected to transverse aortic constriction and six model mice to a pattern of intermittent hypoxia (8 h at 10.5% O2 and 16 h at room air) for 10 days. Twelve mice served as normoxic controls. Faecal samples were obtained and microbiome composition was determined by 16 S rDNA pyrosequencing and bioinformatics analysis by Quantitative Insights into Microbial Ecology.Results A lower abundance of Syntrophobacteraceae was detected in the normoxic-exposed transverse aortic constriction model mice than in controls.The difference between TC and C was of statistical significance (P=0.004), but was P=0.013 between TC and TH.Conclusion sIntermittent hypoxia can alter intestinal flora in a mouse model. Whether this change has influence on the progress of the transverse aortic constriction needs to be further studied.

Key words

intermittent hypoxia / transverse aortic constriction / intestinal flora

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ZHOU Xianjie,LI Jun, LU Feixiang, XIA Jingyuan, and LIU Qingchun. Effect of intermittent hypoxia on transverse aortic constriction of intestinal flora in a murine model[J]. Medical Journal of the Chinese People Armed Police Forces. 2016, 27(11): 1121-1125

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