Objective To develop a PCR method based on 16S rRNA genes for the diagnosis of infections among gynecological and obstetric patients in order to improve the efficiency and accuracy of bacterial detection.Methods 100 patients suspected with blood infections had their blood cultured and bacterial 16S r RNA genes were detected synchronously by extraction of DNA, primer and probe design, PCR amplification and fluorescent quantitative detection of amplified products.The positive rate, sensitivity and specificity of the two Methods were compared.Results The positive rate of PCR was 44% by PCR, which was significantly higher than 15% by blood culture. Withg the criteria of positive blood culture and/or clinical diagnosis of blood infection as control, the sensitivity was 87.9% and the specificity was 90.6% for PCR.Conclusions The positive rate of PCR was significantly higher than that of blood culture and can be used as an early and sensitive index in pathogenic diagnosis of blood infections among gynecological and obstetric patients.
Key words
16S rRNA /
para and grevia /
blood infection /
PCR
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References
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