Effects of sodium butyrate on intestinal micro-vascular permeability and blood flow in rats with severe scald injury

TANG Fubo; ZHENG Jinguang; ZHANG Wenjing; HU Sen; and BAI Xiaodong

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Medical Journal of the Chinese People Armed Police Forces ›› 2016, Vol. 27 ›› Issue (3) : 246-249.

ORIGINAL ARTICLES

Effects of sodium butyrate on intestinal micro-vascular permeability and blood flow in rats with severe scald injury

TANG Fubo^1,2,ZHENG Jinguang¹, ZHANG Wenjing³,HU Sen⁴,and BAI Xiaodong¹

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Abstract

Objective To investigate the small intestine micro-vascular permeability and blood flow protective effects of sodium butyrate on rats following 50%TBSA full-thickness burns. Methods 48 SD rats, weighing 240-260 g, were randomly divided into three groups: sham group, scald group, scald + BTR group. Rats in scald group and scald + BTR group were subjected to 50% TBSA third-degree burns by immersing the back for 15 seconds and the abdomen for 8 seconds in boiling water. The sham group was immersed in 37 ℃ water instead. And then 1ml BTR (400 mg/kg) or 1ml normal saline was intraperitoneally injected, respectively. Blood flow in small intestine was measured by Doppler 3 and 6 hours after injury. Blood was drawn from the heart at 3 and 6 hours after injury for measurement of serum diamine oxidase (DAO); the small intestine tissues were harvested for the measurement of micro-vascular permeability and the changes in tissue water content. Results Compared with scald group, the activity of DAO [(43.76±9.34)U/L vs (73.29±11.34) U/L], the micro-vascular permeability [(4.26±0.98)μg/ml vs (5.53±1.31) μg/ml] and the rate of tissue water [(63.67±3.35)% vs (74.32±3.74)%] in scald + BTR group were significantly lower at 3 hours after scald injury (all P<0.05). In addition, BTR was shown to significantly increase the IMBF level [(67.21±9.47)BPU vs (55.18±10.48)BPU] induced by scald injury at 3 hours (P<0.05). The result at 6 hours after scald injury was similar to 3 hours. Conclusions Sodium butyrate has significant protective effects on small intestinal micro-vascular permeability and blood flow in rats with severe scald injury.

Key words

sodium butyrate / scald / small intestine / tissue water content / vascular permeability

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TANG Fubo,ZHENG Jinguang, ZHANG Wenjing,HU Sen,and BAI Xiaodong. Effects of sodium butyrate on intestinal micro-vascular permeability and blood flow in rats with severe scald injury[J]. Medical Journal of the Chinese People Armed Police Forces. 2016, 27(3): 246-249

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