Objective To study the effect of different training modes on plasma creatine kinase (CK) and cell-free plasma DNA (cf-DNA) in soldiers of PAP and sought sensitive marker of early diagnosis of exercise-induced fatigue and muscle injury in military training. Methods Forty-five soldiers of PAP were randomly divided into three groups: aerobic training group (5 km running), concentric strength training group (push-up and sit-ups) and eccentric strength training group (squatting-jump on-the-spot and leapfrog). Plasma creatine kinase (CK) and cf-DNA were determined pre-training, immediate post-training, 30 min, 1 h, 12 h and 24 h after training, respectively. Results Plasma CK and cf-DNA in aerobic training group were not changed significantly at all time-points (P>0.05). Plasma CK of concentric strength training group was significantly elevated 12 h post-training [(315±95) vs (134±45) IU/L, P<0.01] and recovered at 24 h [(151±71) vs (134±45) IU/L, P>0.05]; cf-DNA was significantly raised immediately after training [(59.1±12.7) vs (43.4±8.0) pg/μl, P<0.05], reached peak value 30 min post-training [(95.3±13.6) vs (43.4±8.0) pg/μl, P<0.01] and restored to base-line at 12 h [(47.7±10.9) vs (43.4±8.0) pg/μl, P>0.05]. Time course changes in plasma CK and cf-DNA of eccentric strength training group were similar to those in concentric strength training group, but elevation extent was significantly higher than that in latter (P<0.01). Conclusions The response of cf-DNA to exercise is dependent on the training modes. Aerobic training of middle intensity does not influence cf-DNA but strength training especially eccentric strength training can significantly impact cf-DNA. cf-DNA may be a sensitive marker for early diagnosis of exercise-induced fatigue and exercise induced muscle injury in military training.
Key words
military training /
training modes /
soldier /
creatine kinase /
cell-free plasma DNA
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Funding
武警部队后勤科研项目(WHKL15-3),武警后勤学院科研创新团队项目(WHTD201308);武警后勤学院教学研究项目(WHJY201313)
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