Transplantation of bone mesenchymal stem cells derived neuron-like cells for treatment of rat spinal cord injury

GAO Feng; ZHANG Xiaofeng; DUAN Yanwei; YAN Huaining; PAN Yongfei; YE Rong; and WEN Li

PDF(2733 KB)

Medical Journal of the Chinese People Armed Police Forces ›› 2016, Vol. 27 ›› Issue (3) : 285-288.

ORIGINAL ARTICLES

Transplantation of bone mesenchymal stem cells derived neuron-like cells for treatment of rat spinal cord injury

GAO Feng, ZHANG Xiaofeng, DUAN Yanwei, YAN Huaining, PAN Yongfei, YE Rong, and WEN Li

Author information +

History +

Abstract

Objective To study transplantation of bone mesenchymal stem cells (BMSCs) derived neuron-like cells for repairing the rat spinal cord injury. Methods BMSCs were isolated from the femur of 4-week-old Sprague-Dawley rats, after culture and proliferation, then differentiated into neuron-like cells induced by retinoic acid (RA). After spinal cord injury (SCI) model preparation, forty-eight rats were randomly assigned to the following three groups (n=18): the neuron-like cells group, the BMSCs group and the control group. The rats’ motor functional recovery was evaluated by BBB score and Rivlin platform test at 2 w, 4 w, 6 w after operation. Results The neuron-like cells derived from BMSCs had some neurocyte characteristics. Transplantation of neuron-like cells(16.56±2.42) showed higher score than BMSCs(13.70±2.66). The rat functional recovery in the neuron-like cells group and the BMSCs group was better than in the control group. There was more nerve fibers with normal structure could be observed in the neuron-like cells group. Conclusions Transplantation of neuron-like cells derived from BMSCs for treating rat spinal cord injury is a promising treatment.

Key words

BMSCs / neuron-like cells / differentiation / spinal cord injury

Cite this article

EndNote

Ris (Procite)

Bibtex

Download Citations

GAO Feng, ZHANG Xiaofeng, DUAN Yanwei, YAN Huaining, PAN Yongfei, YE Rong, and WEN Li. Transplantation of bone mesenchymal stem cells derived neuron-like cells for treatment of rat spinal cord injury[J]. Medical Journal of the Chinese People Armed Police Forces. 2016, 27(3): 285-288

References

[1]Yasutaka O, Kenji T, Yusuke A, et al. Canine bone marrow stromal cells promote functional recovery in mice with spinal cord injury[J]. J Veter Med Sci, 2014, 76(6): 905-908.
[2] Veeravalli K K, Dasari V R, Tsung A J, et al. Human umbilical cord blood stem cells upregulate matrix metalloproteinase-2 in rats after spinal cord injury[J]. Neurobiol Dis, 2009, 36(1): 200-212.
[3] Vaquero J, Zurita M. Bone marrow stromal cells for spinal cord repair: a challenge for contemporary neurobiology [J]. Histol Histopathol, 2009, 24(1):107-116.
[4] 尹小雨,陈刚. 干细胞移植治疗脊髓损伤进展[J]. 中华神经外科疾病研究杂志, 2014, 13(1): 89-91.
[5] Khan T, Havey R M, Sayers S T, et al. Animal models of spinal cord contusion injuries[J]. Lab Anim Sci, 1999, 49(2): 161-172.
[6] Basso D M, Beattie M S, Bresnahan J C. A sensitive and reliable locomotor rating scale for open-field testing in rats[J]. J Neurotrauma, 1995, 12(1): 1-21.
[7] Rivlin A S,Tator C H.Objective clinical assessment of motor function after experimental spinal cord injury in the rat[J].Neurosurg,1977,47(4): 577-581.
[8] Wang L J, Zhang R P, Li J D. Transplantation of neurotrophin-3-expressing bone mesenchymal stem cells improves recovery in a rat model of spinal cord injury [J]. Biomaterials, 2014, 156(7): 1409-1418.
[9] Pal R, Venkataramana N K, Bansal A, et al. Ex vivo-expanded autologous bone marrow-derived mesenchymal stromal cells in human spinal cord injury/paraplegia: a pilot clinical study [J]. Cytotherapy, 2009, 11(7): 897-911.
[10] Zeng Xiang, Qiu XueCheng, Ma YuanHuan, et al.I ntegration of donor mesenchymal stem cell-derived neuron-like cells into host neural network after rat spinal cord transaction [J]. Biomaterials, 2015, 53: 184-201.
[11] Crigler L, Robey R C, Asawachaicharn A, et al. Human mesenchymal stem cell subpopulations express a variety of neuro-regulatory molecules and promote neuronal cell survival and neuritogenesis [J].Exp Neurol,2006,198(1):54-64.
[12] 朱天琦,魏明发. 间充质干细胞体外分化为神经样细胞的研究进展[J]. 中华小儿外科杂志,2014,35(1):72-75.
[13] Okada Y, Shimazaki T,Sohue G, et al. Retinoic-acid-concentration-dependent acquisition of neural cell identity during in vitro differentiation of mouse embryonic stem cells [J].Dev Biol,2004,275(1):124-142.
[14] Munoz J L, Greco S J,Patel SA,et al.Feline bone marrow derived mesenchymal stromal cells(MSCs) show similar phenotype and functions with regards to neuronal differentiation as human MSCs [J].Differentiation,2012,84(2):214-222.
[15] Jiang X,Cao H Q,Shi L Y,et al.Nanofiber topography and sustained biochemical signaling enhance human mesenchymal stem cell neural commitment[J].Acta Biomater,2012,8(3):1290-1302.
[16] 李鹏斌, 郑启新, 吴永超. 骨髓基质干细胞定向分化为神经细胞及分化后细胞功能特征的研究[J]. 中华创伤骨科杂志, 2012, 12(4): 355-359.
[17] 张德龙,王伟,张力,等. Wnt/β-catenin信号途径促进肌源性干细胞向神经细胞分化[J]. 解放军医学院学报, 2014, 35(4): 383-387.