Association of genetic polymorphisms in SLC22A1 and SLC22A2 with intolerance to metformin in type 2 diabetes

FU Ting; SHI Jiandang; KUANG Xia; and WANG Suli

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Medical Journal of the Chinese People Armed Police Forces ›› 2016, Vol. 27 ›› Issue (5) : 456-459.

Orignal Article

Association of genetic polymorphisms in SLC22A1 and SLC22A2 with intolerance to metformin in type 2 diabetes

FU Ting^1,2,SHI Jiandang³,KUANG Xia²,and WANG Suli^1,2

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Abstract

Objective To study the relationship between gene polymorphisms of 1222A＞G in SLC22A1 and 808G＞T in SLC22A2 with the gastrointestinal side-effects in metformin-treated type 2 diabetes patients.Methods One hundred and thirty type 2 diabetes patients treated with metformin at the first time were recruited.Genomic DNA samples were extracted from the patients’ leukocytes in the peripheral blood.The genotypes were determined by the allele-specific polymerase chain reaction(AS-PCR)and gel electrophoresismethod.Results Three genotypes of AA, AG, GG were found in SLC22A11222A＞G site and the mutation frequency was 64.62%.An allele of 1222A＞G was associated with the incidence of gastrointestinal side-effects of metformin(P＜0.05).Three genotypes of GG, GT, TT were found in SLC22A2 808G＞T site and the mutation frequency was 11.15%.The incidence of gastrointestinal side effects in metformin-treated type 2 diabetes patients with different genotypes had no significant differences(P＝0.559).The patients in metformin intolerance group were older than the patients in metformin tolerance group. The patients in metformin intolerance group had higher systolic blood pressure than the patients in metformin tolerance group.Conclusions SLC22A11222A＞G gene polymorphis was associated with the incidence of gastrointestinal side effects in metformin-treated type 2 diabetes patients, however SLC22A2 808G＞T is not.Older age and high systolic blood pressure may be the risk factors for metformin intolerance.

Key words

metformin / SLC22A1 / SLC22A2 / gene polymorphisms / gastrointestinal side-effects

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FU Ting,SHI Jiandang,KUANG Xia,and WANG Suli. Association of genetic polymorphisms in SLC22A1 and SLC22A2 with intolerance to metformin in type 2 diabetes[J]. Medical Journal of the Chinese People Armed Police Forces. 2016, 27(5): 456-459

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