Construction of a new tissue engineering skin detection model using HaCaT cells

LI Yan; ZHOU Zhoulin; LU Tao; and  LI Ruixin

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Medical Journal of the Chinese People Armed Police Forces ›› 2016, Vol. 27 ›› Issue (7) : 672-675.

Orignal Article

Construction of a new tissue engineering skin detection model using HaCaT cells

LI Yan^1,2, ZHOU Zhoulin^1,3, LU Tao¹,and LI Ruixin⁴

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Abstract

Objective To construct human immortalized keratinocytes HaCaT as seed cells for tissue engineering skin model.Methods A novel tissue engineered skin model was constructed for the detection of human immortalized keratinocytes and fibroblasts as seed cells. 10 kinds of chemicals were selected as test materials, the surface of local contact skin model was 15 min, and the relative viability of cells was measured by MTT colorimetry.Results Three different batches of the model, based on the relative values of cell viability as a decision, the new detection model constructed could determine eight kinds of harsh chemicals,after addition of IL-1α experiment improved the accuracy of the model, the accuracy rate was 75%.Conclusions This new model provides an effective in vitro test results and prove the feasibility of the model. At the same time it should use a greater number of substances for further validation and tests in different lab oratories to properly assess reproducibility.

Key words

HaCaT cells / tissue-engineered skin / fibroin / irritant / chemicals

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LI Yan, ZHOU Zhoulin, LU Tao,and LI Ruixin. Construction of a new tissue engineering skin detection model using HaCaT cells[J]. Medical Journal of the Chinese People Armed Police Forces. 2016, 27(7): 672-675

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