Establishing the model of acute ischaemic left heart failure with mitral regurgitation in beagles

SUN Jing; FAN Chunzhi; DOU Jianping; and WEN Chaoyang

PDF(874 KB)

Medical Journal of the Chinese People Armed Police Forces ›› 2016, Vol. 27 ›› Issue (9) : 906-909.

ORIGINAL ARTICLES

Establishing the model of acute ischaemic left heart failure with mitral regurgitation in beagles

SUN Jing¹, FAN Chunzhi², DOU Jianping³, and WEN Chaoyang⁴

Author information +

History +

Abstract

Objective To establish the model of acute ischaemic left heart failure with mitral regurgitation in beagles. Methods (1)The construction of mitral regurgitation (MR)animal model: a catheter was introduced into left ventricle through the carotid artery in 15 experiment beagles, then a biopsy clamp was put into the mitral valve nearby through the catheterization and chordae tendineae was pinched under the guidance of echo in order to construct mitral regurgitation.(2) The construction of acute ischaemic left ventricular (LV) diastolic heart failure: 15 beagles with MR were further processed with coronary microembolization by microspheres repeatedly. Results (1) All mitral regurgitation models were successfully produced. The chordae tendineaes were broken in thirteen dogs, causing moderate MR. One dog with natural mild MR was with broken chordae tendineae. Continuous wave Doppler showed that the MR peak velocity was (483±1.12) cm/s, the maximun pressure gradient was (162.7±1.8) mmHg. (2)12 models of acute ischaemic left diastolic heart failure with mitral regurgitation were established. Left ventricular end-diastolic pressure emitting equal or greater than 5 mmHg was defined as model successful. The left ventricular end-diastolic pressure (LVEDP), -dP/dtmax, Tau-c in successful model were increased significantly (P＜0.05). Conclusions This method is safe, feasible and easily successful in constructing the MR animal model under the guidance of transthoracic echocardiography.Coronary microembolization with microspheres is a sueeessfull way for establishing the acute ischaemic left diastolic heart failure.

Key words

guiding of transthoracic echocardiography / MR / acute myocardial ischaemic / left ventricular (LV) diastolic failure / beagles animal model

Cite this article

EndNote

Ris (Procite)

Bibtex

Download Citations

SUN Jing, FAN Chunzhi, DOU Jianping, and WEN Chaoyang. Establishing the model of acute ischaemic left heart failure with mitral regurgitation in beagles[J]. Medical Journal of the Chinese People Armed Police Forces. 2016, 27(9): 906-909

References

[1] Weiss J L, Frederiksen J W, Weisfeldt M L, et al. Hemodynamic determi-nants of the time-course of fall in canine left ventricular pressure [J]. J Clin Invest, 1976,58: 751-760.
[2] Kleaveland J P, Kussmaul W G, Vinciguerra T, et al. Volume overload hypertrophy in a closed-chest model of mitral regurgitation [J]. Am J Physiol, 1988, 254(6 Pt 2): H1034-H1041.
[3] 李凯,罗富良,田毅,等. 比格犬二尖瓣反流慢性心力衰竭模型的建立[J]. 中国循环杂志, 2014, 29(增刊):37.
[4] Pierpont M E, Foker J E, Pierpont G L. Myocardial carnitine metabolism in congestive heart failure induced by incessant tachycardia [J]. Basic Res Cardiol, 1993, 88(4): 362-370.
[5] Igawa A, Nozawa T, Yoshida N,et al. Heterogeneous cardiac sympathetic innervation in heart failure after myocardial infarction of rats [J]. Am J Physiol Heart Circ Physiol, 2000, 278(4): H1134-H1141.
[6] 刘宝辉,李明凯,罗晓星,等.心力衰竭实验动物模型构建方法研究[J]. 中国心血管杂志, 2013, 18(3): 233-236.
[7] 李彩红,王永刚,罗文平,等.慢性心力衰竭动物模型的制作进展[J]. 长春中医药大学学报, 2015, 31(3): 647-650.
[8] 苏强,李浪,马国添,等.猪急性冠状动脉微栓塞模型的建立[J]. 中国动脉硬化杂志, 2014, 22(4): 335-339.
[9] New York Heart Association. New concepts in diastolic dysfunction and diastolic heart failure. Part II: causal mechanisms and treatment [J]. Circulation, 2002,105(12): 1503-1508.
[10] Lew Y W. Evaluation of left ventricular disatolic function [J]. Circulation, 1989, 79(6):1393-1397.
[11] 张庆勇,王志华,李晓波,等.介入法建立长期可随访冠状动脉微栓塞动物模型[J]. 介入放射学杂志, 2009, 18(12): 920-922.
[12] Smiseth O A. A reproducible and stable model of acute ischaemic left ventricular failure in dogs [J]. Clin Physiol, 1982, 2(3):225-239.
[13] Houser S R, Margulies K B, Murphy A M, et al. Animal models of heart failure: a scientific statement from the American Heart Association.[J]. Circul Res, 2012, 111(1):131-150.
[14] Alpert N R, Mulieri L A, Warshaw D. The failing human heart[J]. Cardiovasc Res, 2002,54(1): 1-10.
[15] Schmitto J D, Ortmann P, Wachter R. Chronic heart failure induced by multiple sequential coronary microembolization in sheep[J]. Int J Artif Organs, 2008, 31(4): 348-353.
[16] Schmitto J D, Coskun K O, Coskun S T. Hemodynamic changes in a model of chronic heart failure induced by multiple sequential coronary microembolization in sheep[J]. Artif Organs, 2009, 33(11): 947-952.
[17] He K L, Dickstein M, Sabbah H N. Mechanisms of heart failure with well preserved ejection fraction in dogs following limited coronary microembolization[J]. Cardiovasc Res, 2004, 64(1): 72-83.