Lactobacillus plantarum prevents bacterial translocation induced by intestinal ischemia and reperfusion injury in rats

LI Jingjing; YOU Huanhuan; WANG Dan; ZHAO Yongfeng; and WANG Bin

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Medical Journal of the Chinese People Armed Police Forces ›› 2017, Vol. 28 ›› Issue (3) : 240-243.

Lactobacillus plantarum prevents bacterial translocation induced by intestinal ischemia and reperfusion injury in rats

LI Jingjing¹, YOU Huanhuan¹, WANG Dan¹, ZHAO Yongfeng¹, and WANG Bin²

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Abstract

Objective To investigate the ability of Lactobacillus plantarum L2 to prevent intestinal ischemia and reperfusion induced bacterial translocation.Methods 32 SD rats were randomly divided into four groups: sham, Lactobacillus intragastric administration, ischemia and reperfusion (I/R) model, Lactobacillus pre-treatment and I/R model. The bacterial translocation, changes of gut microflora, levels of proinflammatory cytokines in plasma and intestinal morphology were observed.Results The increase in bacterial translocation was significant after 4 h I/R treatment (P<0.01) as compared with sham-operated group. There were obvious pathological changes of mucosal morphology in I/R rats and the number of anaerobic bacteria such as Bifidobacteria, Lactobacillus and Bacteroides was significantly reduced. The level of TNF-α in plasma was increased after I/R injury. Lactobacillus plantarum treatment alone had no effect on intestinal barrier function. However, pretreatment of rats with Lactobacillus plantarum L2 significantly abrogated I/R induced intestinal mucosal injury and prevented translocation of bacteria to mesenteric lymph nodes or to remote organs.Conclusions Lactobacillus plantarum L2 has the ability to prevent bacterial translocation and attenuate I/R induced intestinal injury.

Key words

Lactobacillus plantarum / prevent / gut / ischemia and reperfusion / bacterial translocation

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LI Jingjing, YOU Huanhuan, WANG Dan, ZHAO Yongfeng, and WANG Bin. Lactobacillus plantarum prevents bacterial translocation induced by intestinal ischemia and reperfusion injury in rats[J]. Medical Journal of the Chinese People Armed Police Forces. 2017, 28(3): 240-243

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