Inhibitory effect of Gongxuening on myometrial contractions and  mechanisms

FU Tao; WANG Limei; ZHAO Zhenhu; SHAN Yajun; LIU Xiaolan; and CONG Yuwen

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Medical Journal of the Chinese People Armed Police Forces ›› 2017, Vol. 28 ›› Issue (6) : 557-559.

ORIGINAL ARTICLES

Inhibitory effect of Gongxuening on myometrial contractions and mechanisms

FU Tao¹, WANG Limei², ZHAO Zhenhu², SHAN Yajun², LIU Xiaolan², and CONG Yuwen²

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Abstract

Objective To investigate the mechanism underlying myometrial contraction stimulated by Gongxuening, and to provide reference for rational use of Gongxuening in the treatment of abnormal uterine bleeding.Methods The contraction of the uterus in vitro induced by Gongxuening in rats was observed using isolated uterine tension measurement, and the influence of such potential factors as epinephrine and its receptor modulators on the contraction was studied.Results It was found that epinephrine at the dose of 0.001μg/ml completely inhibited rat myometrial contractions induced by 50 μg/ml Gongxuening, while β₁ adrenergic receptor (β₁-AR) antagonists atenolol and metoprolol both completely reversed the inhibition by epinephrine. α-AR antagonist phentolamine could not reverse the inhibition by epinephrine during rat myometrial contractions induced by Gongxuening, although it completely inhibited myometrial contractions induced by α-AR agonist methoamine.Conclusions The possible mechanism of rat myometrial contractions induced by Gongxuening may be through inhibition of epinephrine β- AR activation.

Key words

Gongxuening / myometrial contractions / epinephrine / adrenergic receptor

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FU Tao, WANG Limei, ZHAO Zhenhu, SHAN Yajun, LIU Xiaolan, and CONG Yuwen. Inhibitory effect of Gongxuening on myometrial contractions and mechanisms[J]. Medical Journal of the Chinese People Armed Police Forces. 2017, 28(6): 557-559

References

[1] 范光升,庄留琪.宫血宁胶囊减少药物流产后阴道出血的有效性和安全性研究[J].中国妇幼保健, 2010, 25(35): 5225-5226.
[2] 张胜利, 吴尚纯. 宫血宁治疗置宫内节育器后异常出血疗效的Meta分析 [J]. 中国计划生育学杂志, 2010, 18(8): 458-459.
[3] Dupuis M, Lévy A, Mhaouty-Kodja S. Functional coupling of rat myometrial alpha 1-adrenergic receptors to Gh alpha/tissue transglutaminase 2 during pregnancy [J]. J Biol Chem, 2004, 279(18): 19257-19263.
[4] Tanaka Y, Horinouchi T, Koike K. New insights into beta-adrenoceptors in smooth muscle: distribution of receptor subtypes and molecular mechanisms triggering muscle relaxation [J]. Clin Exp Pharmacol Physiol, 2005,32(7):503-14.
[5] 赵振虎,李建勇,善亚军,等.宫血宁对大鼠离体子宫的节律收缩作用 [J].　解放军药学学报,2004,20(2):93-95.
[6] 赵振虎,李建勇,苑晓玲, 等. 皂苷提取物宫血宁缩宫作用与细胞外钙内流关系的研究[J].中国药物与临床, 2005, 5(6): 415-417.
[7] 苏佳,刘泽源,赵振虎,等.PLA2 /AA信号途径与重楼皂苷诱导大鼠子宫平滑肌收缩活动的关系研究[J]. 军事医学科学院院刊, 2008, 32(3): 264-268.
[8] Guo L, Su J, Deng B W, et al. Active pharmaceutical ingredients and mechanisms underlying phasic myometrial contractions stimulated with the saponin extract from Paris polyphylla Sm. var. yunnanensis used for abnormal uterine bleeding [J]. Hum Reprod,2008,23(4):964-971.
[9] Wang L, Jia C, Yu Z, et al. Pennogenin tetraglycoside induces rat myometrial contraction and MLC20 phosphorylation via PLC-IP(3) and RhoA/Rho kinase signaling pathways [J]. PLoS One, 2012, 7(12):e51536.
[10] Martínez M I, Esta L, Morales-Olivas F J, et al. Effect of histamine and histamine analogues on human isolated myometrial strips [J]. Br J Pharmacol, 1992 , 107(2): 528-531.
[11] Darios E S, Seitz B, Watts S W.Smooth muscle pharmacology in the isolated virgin and pregnant rat uterus and cervix [J]. J Pharmacol Exp Ther,2012,341(3):587-596.
[12] Liu Y L, Nwosu U C, Rice P J. Relaxation of isolated human myometrial muscle by beta2-adrenergic receptors but not beta1-adrenergic receptors [J]. Am J Obstet Gynecol, 1998, 179(4): 895-898.
[13] Jovanovic P, Stefanovic B, Spasojevic N, et al. Effects of oxytocin on adreno-medullary catecholamine synthesis, uptake and storage in rats exposed to chronic isolation stress [J]. Endocr Res, 2016, 41(2): 124-131.