Effect and mechanism of sliencing S100A4 by small RNA interference of S100A13 on thyroid cells

LI Jian; YAN Shuangtong; GONG Yanping; LU Yanhui; SUN Banruo; CHENG Xiaoling; SHAO Yinghong

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Medical Journal of the Chinese People Armed Police Forces ›› 2018, Vol. 29 ›› Issue (10) : 960-963.

ORIGINAL ARTICLES

Effect and mechanism of sliencing S100A4 by small RNA interference of S100A13 on thyroid cells

LI Jian¹, YAN Shuangtong¹, GONG Yanping¹, LU Yanhui¹, SUN Banruo¹, CHENG Xiaoling¹, SHAO Yinghong²

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Abstract

Objective To investigate the effect of interference with the expression of calcium binding protein A13 (S100A13) on the invasion and migration of thyroid carcinoma TPC-1 cells and the possible mechanism.Methods The expression of S100A13 gene in thyroid cancer TPC-1 cells was knocked down by siRNA. The effect of S100A13 on the migration of TPC-1 cells was evaluated with the wound healing scratch test and Transwell assay. The effect of S100A13 on the invasiveness of TPC-1 cells was assessed by Transwell invasion test. The expressions of S100A13, MMP-2, E-cadherin and vimentin were detected by Western Blot.Results Compared with the control group, the silencing of S100A13 by siRNA in human thyroid carcinoma TPC-1 cells significantly increased the scratch width(25.214±2.687 vs 69.223±5.291), but the ability of invasion(0.897±0.082 vs 0.521±0.053) and migration(1.263±0.252 vs 0.759±0.062)decreased significantly (P<0.05). The levels of MMP-2 (0.523±0.062 vs 0.246±0.033)and vimentin(0.863±0.082 vs 0.458±0.053) in S100A13-treated cells were significantly decreased (P<0.05), while E-cadherin protein(0.445±0.053 vs 0.755±0.078)was significantly increased (P<0.05).Conclusions Interference with S100A13 expressions by siRNA inhibits the invasion and migration of thyroid carcinoma TPC-1 cells, which may be related to the inhibition of EMT and MMP-2.

Key words

thyroid cancer / S100A13 / invasion / migration

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LI Jian, YAN Shuangtong, GONG Yanping, LU Yanhui, SUN Banruo, CHENG Xiaoling, SHAO Yinghong. Effect and mechanism of sliencing S100A4 by small RNA interference of S100A13 on thyroid cells[J]. Medical Journal of the Chinese People Armed Police Forces. 2018, 29(10): 960-963

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