Objective To explore the effect of quercetin on the proliferation and expression of HIF-1α and VEGF , and the mechanisms in hypoxia hepatoma carcinoma cell HepG2.Methods The cells were divided into normal control group , hypoxia group and hypoxia combined with quercetin group. A hypoxia model was established using cobalt chloride (CoCl2).The proliferation of cells was determined by MTT assay.The mRNA expression and protein expression of HIF-1α and VEGF were determined by real-time quantification PCR and Western blot.Results Compared with the normal control group, the proliferation of the hypoxia group was decreased at 24, 48 and 72 h(P<0.05),while the proliferation in hypoxia combined with quercetin group was decreased at 24,48 and 72 h(P<0.05)compared with the hypoxia group.The optical density was 0.640±0.016、1.287±0.025 and 1.738±0.027 respectively in normal control group, 0.551±0.021、0.851±0.028 and 1.268±0.018 in hypoxia group, and 0.435±0.023、0.717±0.013 and 0.984±0.037 respectively in hypoxia combined with quercetin group.The mRNA and protein expressions of HIF-1α and VEGF in hypoxia group were increased(P<0.05)compared with normal control group. Quercetin down-regulated the mRNA and protein expressions of VEGF(P<0.05)in hypoxia cells,but had no effect on the mRNA and protein expression of HIF-1α (P>0.05).The mRNA expressions of HIF-1α and VEGF were both 1.000±0.000 and the protein expressions were 0.616±0.016 and 0.831±0.034 in normal control group. The protein expressions were 1.385±0.047 and 2.022±0.076 in the hypoxia group, but were 1.406±0.029 and1.137±0.054 in the hypoxia combined with quercetin group.Results Quercetin can inhibit the proliferation of hepatoma carcinoma cell HepG2 under hypoxia conditions,which may be related to down-regulated expressions of VEGF.
Key words
quercetin /
hepatoma carcinoma cell /
hypoxia /
HIF-1α /
VEGF
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