Objective To select chemotherapeutic agents based on in vitro chemosensitivity test of glioma in tissue culture in order to provide experimental data for individualized treatment of glioma.Methods One hundred and twelve specimens resected from brain glioma were used to evaluate the in vitro chemosensitivity of glioma with tissue culture. Six chemotherapeutic agent groups against brain cancer were designed, which were nimustine (ACNU), cisplatin (DDP), temozolomide (TMZ) , vincristine (VCR), teniposide (VM26) and DDP＋VM26. The ultrastructural changes of brain glioma after exposure to chemotherapeutic agents were observed under an electron microscope. Furthermore, the correlations between drug chemosensitivity and patients’ pathological grade were analyzed.Results The chemosensitivity of DDP＋VM26 group and VM26 group was significantly higher than that of the other four groups (P＜0.003). Under an electron microscope, the glioma cells showed apoptosis and necrosis changes after exposure to chemotherapeutic agents. The chemosensitivity of DDP＋VM26 group was correlated with the pathological grade of patients (χ2=4.447, P=0.035).Conclusions The sensitivity of patients with different glioma to chemotherapeutic drugs is different, and the apoptosis or necrosis of tumor cells may be one of the mechanisms of chemotherapeutic drugs.
Key words
glioma /
in vitro chemosensitivity test /
tissue culture /
electron microscope
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