Biological imaging effect and biocompatibility of graphene quantum dots (GQDs) of different size in PC12 cells

DENG Banglian; CHEN Xuefeng; MENG Lei; YI Zian; QIN Wen; LIU Yan; SONG Shuang; PENG Chao; and JU Yanjing.

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Medical Journal of the Chinese People Armed Police Forces ›› 2018, Vol. 29 ›› Issue (9) : 901-904.

ORIGINAL ARTICLES

Biological imaging effect and biocompatibility of graphene quantum dots (GQDs) of different size in PC12 cells

DENG Banglian^1,2, CHEN Xuefeng¹, MENG Lei¹, YI Zian¹, QIN Wen³, LIU Yan³, SONG Shuang⁴, PENG Chao¹, and JU Yanjing¹.

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Abstract

Objective To evaluate the biological imaging effect and biocompatibility of graphene quantum dots (GQDs) of different size in PC12 cells.Methods The hydrodynamic size and zeta potencial of GQDs in distilled water (DW) were determined by dynamic light scattering (DLS). Furthermore, cell survival rates following treatment with GQDs were evaluated using the CCK-8 assay and the biological imaging effect of GQDs in PC12 cells was observed under a confocal microscope.Results The toxic effect of GQDs in PC12 cells was size-dependent. The results of CCK-8 assay indicated that 15 nm GQDs showed lower toxicity than CDs in PC12 cells. After PC12 cells were incubated 48 h with 500 μg/ml GQDs, cell viability remained at 80% or more. What’s more, 15 nm GQDs could be taken up by PC12 cells more easily and more than 80% cells could be labeled successfully, which indicated good biological imaging effects.Conclusions GQDs have low cytotoxicity and excellent biological imaging properties, which are a suitable nanomaterial for neuroimaging.

Key words

graphene quantum dots / PC12 cells / biological imaging / cytotoxicity

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DENG Banglian, CHEN Xuefeng, MENG Lei, YI Zian, QIN Wen, LIU Yan, SONG Shuang, PENG Chao, and JU Yanjing.. Biological imaging effect and biocompatibility of graphene quantum dots (GQDs) of different size in PC12 cells[J]. Medical Journal of the Chinese People Armed Police Forces. 2018, 29(9): 901-904

References

[1] Michalet X, Pinaud F F, Bentolila L A, et al. Quantum dots for live cells, in vivo imaging, and diagnostics[J]. Science, 2005, 307(5709): 538-544.
[2] Xu Y, Wang X, Zhang W L, et al. Recent progress in two-dimensional inorganic quantum dots[J]. Chem Soc Rev, 2018, 47(2): 586-625.
[3] Li L, Wu G, Yang G, et al. Focusing on luminescent graphene quantum dots: current status and future perspectives[J]. Nanoscale, 2013, 5(10): 4015-4039.
[4] Yoo J M, Kang J H, Hong B H. Graphene-based nanomaterials for versatile imaging studies[J]. Chem Soc Rev, 2015, 44(14): 4835-4852.
[5] Tan D, Zhou S, Qiu J. Comment on “Upconversion and Downconversion Fluorescent Graphene Quantum Dots: Ultrasonic Preparation and Photocatalysis” [J]. Acs Nano, 2012, 6(8):1059-1064.
[6] Xu G, Zeng S, Zhang B, et al. New generation cadmium-free quantum dots for biophotonics and nanomedicine[J]. Chem Rev, 2016, 116(19):12234.
[7] Halamodakenzaoui B, Ceridono M, Colpo P, et al. Dispersion behaviour of silica nanoparticles in biological media and its influence on cellular pptake[J]. PLoS One, 2015,10(10):0141593.
[8] Markovic Z M, Ristic B Z, Arsikin K M, et al. Graphene quantum dots as autophagy-inducing photodynamic agents[J]. Biomaterials, 2012,33(29):7084-7092.
[9] Li P, Xu T, Wu S, et al. Chronic exposure to graphene-based nanomaterials induces behavioral deficits and neural damage in Caenorhabditis elegans[J]. J Appl Toxicol, 2017,37(10):1140-1150.
[10] Wu C, Wang C, Han T, et al. Insight into the cellular internalization and cytotoxicity of graphene quantum dots[J]. Adv Healthc Mater, 2013,2(12):1613-1619.
[11] Yuan X, Liu Z, Guo Z, et al. Cellular distribution and cytotoxicity of graphene quantum dots with different functional groups[J]. Nanoscale Res Lett, 2014, 9(1):108.
[12] Eda G, Lin Y Y, Mattevi C, et al. Blue photoluminescence from chemically derived graphene oxide[J]. Adv Mater, 2010, 22(4):505.
[13] Agarwal R, Singh V, Jurney P, et al. Mammalian cells preferentially internalize hydrogel nanodiscs over nanorods and use shape-specific uptake mechanisms[J]. Pnas, 2013,110(43):17247-17252.
[14] Asati A, Santra S, Kaittanis C, et al. Surface-charge-dependent cell localization and cytotoxicity of cerium oxide nanoparticles[J]. Acs Nano, 2010,4(9):5321-5331.
[15] Zhu J, Liao L, Zhu L, et al. Size-dependent cellular uptake efficiency, mechanism, and cytotoxicity of silica nanoparticles toward HeLa cells[J]. Talanta, 2013,107:408-415.
[16] Soenen S J, Rivera-Gil P, Montenegro J M, et al. Cellular toxicity of inorganic nanoparticles: common aspects and guidelines for improved nanotoxicity evaluation[J]. Nano Today, 2011,6(5):446-465.
[17] Thorek D L, Tsourkas A. Size, charge and concentration dependent uptake of iron oxide particles by non-phagocytic cells[J]. Biomaterials, 2008, 29(26): 3583-3590.