Objective To establish a method for the determination of ferulic acid and glycyrrhizic acid in Xiao Yao Wan.Methods The separation was performed on an Agilent Zorbax XDB-C18 column(150 mm×4.6 mm,5 μm)with acetonitrile/ phosphoric acid solution(0.05%) as the mobile phase with gradient elution at the flow rate of 1.0 ml/min. The detection wavelength was set at 278 nm, and the column temperature was 35 ℃.Results The linear range of ferulic acid and glycyrrhizic acid was 0.054-0.596 μg (r=0.9995) and 0.110-1.217 μg (r=0.9993) respectively. The average recovery was 99.29％(RSD=1.28％, n=6) and 98.34％(RSD=1.61％,n=6) respectively.Conclusions The established HPLC method has proved to be user-friendly, reproducible, precise and suitable for the content determination of Xiao Yao Wan.
Key words
Xiao Yao Wan /
HPLC /
content determination
{{custom_sec.title}}
{{custom_sec.title}}
{{custom_sec.content}}
References
[1] 徐 宇,谈 红,万 新.用RP-HPLC同时测定逍遥丸中当归、芍药的指标成分的含量[J].中国药学杂志,2001,36(8):552-554.
[2] 任爱萍,陈润泽.逍遥丸治疗老年女性抑郁症伴有自主神经功能失调93例[J].陕西中医 2014,35(9):1203-1205.
[3] 秦悦农,鲍以嘉,胡升芳.逍遥丸联合生脉注射液对乳腺癌患者围手术期应激反应的影响[J].中国药业,2015,24(17):21-22.
[4] 张鸿宇,罗 晓.左归丸合逍遥丸治疗围绝经期综合征[J].中国实验方剂学杂志,2012,18(12):295-297.
[5] 国家药典委员会.中华人民共和国药典[S].2015年版,第一部:1354-1358.
[6] 刘幸平,崔小兵,赵晓莉. 高效液相色谱法测定逍遥丸中阿魏酸的含量[J].南京中医药大学学报(自然科学版),2000,16(5):300-301.
[7] 韩慧琴,王晓青,尹贻珍.高效液相色谱法测定逍遥丸(水丸)中甘草苷含量[J].中国药业,2012,21(13):21-22.
[8] 苏兰宜,肖庆青.同时检测逍遥丸中芍药苷和槲皮素的含量研究[J].亚太传统医药,2012,8(8):48-50.
PDF(781 KB)
