Objective To investigate the effect of exercise preconditioning on mitochondrial biogenesis in the rat hippocampus after acute hypobaric hypoxia.Methods Rats were randomly divided into three groups: normal control group (NC), acute hypoxia group (AH), and exercise preconditioning+ acute hypoxia group (EP+AH). Rats in the EP+AH group underwent six weeks of treadmill training on the plain at a speed of 17 m/min, -5° grade for 60 min/day. Rats in AH and EP+AH groups were placed in a hypobaric hypoxia chamber at 0.06 MPa pressure and 10%±2% oxygen for 8 consecutive hours. The mtDNA copies, mitochondrial membrane potential, ROS generation rate, ATP synthesis activity, and the protein expression of PGC-1α, NRF-1, Tfam were measured.Results The mtDNA copies, ROS generation rate, PGC-1αprotein expression, and mitochondrial membrane potential in the AH group were 1.69±0.20, (9.49±1.25) pmol/(min·mg protein), 189.24±21.77, and 4.51±0.65 respectively, compared with 1.00±0.13, (4.83±0.66) pmol/(min·mg protein), 100.00±12.90, and 8.27±1.02 in the NC group respectively. The difference was statistically significant (P＜0.05 or P＜0.01) . The mtDNA copies, ROS generation rate, PGC-1αprotein expression, and mitochondrial membrane potential in the EP+AH group were 1.19±0.15, (6.01±0.93) pmol/(min·mg protein), 141.95±18.12, and 7.02±1.10, respectively, compared with 1.69±0.20, (9.49±1.25) pmol/(min·mg protein), 189.24±21.77, and 4.51±0.65 in the AH group respectively. The difference was also statistically significant (P＜0.05).Conclusions Exercise preconditioning can inhibit acute hypobaric hypoxia induced by mitochondrial biogenesis and oxidative stress while improving mitochondrial energy metabolism in the hippocampus.
Key words
exercise preconditioning /
hypoxia /
hippocampus /
mitochondrial biogenesis /
energy metabolism
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