Comparison of profiles between c9orf72-mutant amyotrophic lateral sclerosis and CHMP2B amyotrophic lateral sclerosis based on bioinformatics analysis

LIN Qipeng; ZHU Qian; and MA Liwei

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Medical Journal of the Chinese People Armed Police Forces ›› 2019, Vol. 30 ›› Issue (2) : 124-127.

Comparison of profiles between c9orf72-mutant amyotrophic lateral sclerosis and CHMP2B amyotrophic lateral sclerosis based on bioinformatics analysis

LIN Qipeng¹, ZHU Qian¹,and MA Liwei²

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Abstract

Objective To compare the difference of gene expressions between C9orf72 mutation ALS and CHMP2B mutation ALS.Methods Datasets (GSE68605 and GSE19332) were acquired from the gene expression omnibus (GEO) database. R software (version 3.5.0), Cytoscape 3.6.1 software and online tool DAVID and STRING were used for data analysis.Results A total of eleven independent samples (eight samples of C9orf72 mutation ALS and three samples of CHMP2B mutation ALS) were analyzed. Thirteen differentially expressed genes (DEGs) were identified in the cervical spinal cord motor neurons of the two distinct ALS variants. It was found that only CALM2, CALM1, CALM3 and RYR2 were classified during GO and KEEG functional enrichment, and that CALM1, CALM2 and CALM3 were outstanding in the Hub protein analysis.Conclusions The expression of CALM genes is up-regulated in the C9orf72 mutation ALS. Calmodulin might be a potential biomarker for ALS diagnosis and effective target for ALS treatment.

Key words

amyotrophic lateral sclerosis / C9orf72 / CHMP2B / CALM / calmodulin

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LIN Qipeng, ZHU Qian,and MA Liwei. Comparison of profiles between c9orf72-mutant amyotrophic lateral sclerosis and CHMP2B amyotrophic lateral sclerosis based on bioinformatics analysis[J]. Medical Journal of the Chinese People Armed Police Forces. 2019, 30(2): 124-127

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