Combination of dihydroartemisinin and gefitinib inhibits cell cycle and migration of lung adenocarcinoma cells in vitro

SONG Fanghua; LUO Meng; FAN Ning; ZHOU Jiajing; LI Yan

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Medical Journal of the Chinese People Armed Police Forces ›› 2019, Vol. 30 ›› Issue (3) : 228-232.

Combination of dihydroartemisinin and gefitinib inhibits cell cycle and migration of lung adenocarcinoma cells in vitro

SONG Fanghua¹, LUO Meng², FAN Ning², ZHOU Jiajing²,LI Yan²

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Abstract

Objective To explore the combined effect of dihydroartemisinin (DHA) and gefitinib (GEF) in lung adenocarcinoma cell lines.Methods Lung adenocarcinoma cell lines A549 and SPC-A-1 were treated with 10μM DHA and (or) 50μM GEF. MTT assay was used to detect cell proliferation, while flow cytometry was adopted to determine cell cycle. Cell migration was observed with cell scratch tests. The effects of DHA and GEF alone or in combination on the expressions of proteins related to cycle and migration were detected by Western blot.Results MTT results showed that DHA combined with GEF(Com group) inhibited the proliferation of A549 and SPC-A-1 cell lines more significantly than in control and single drug groups and in a time-dependent manner (P<0.05). Flow cytometry with PI staining showed that cell cycles were blocked at G0/G1 phase. Western blot showed that the expressions of CDK4 and Cyclin D1 in the Com group decreased more significantly than in control and single drug groups. Cell fusion rate of Com group was also significantly lower than that of control and single drug groups in cell scratch test and the expressions of MMP2 and MMP9 were decreased. The difference was statistically significant (P<0.05).Conclusions DHA combined with GEF can significantly inhibit the proliferation and migration of lung adenocarcinoma cells and block cell cycle.

Key words

dihydroartemisinin / Gefitinib / lung adenocarcinoma / cell cycle / migration

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SONG Fanghua, LUO Meng, FAN Ning, ZHOU Jiajing,LI Yan. Combination of dihydroartemisinin and gefitinib inhibits cell cycle and migration of lung adenocarcinoma cells in vitro[J]. Medical Journal of the Chinese People Armed Police Forces. 2019, 30(3): 228-232

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