Downregulation of p53 promotes cell proliferation and migration and inhibits apoptosis by targeting signal transducers and activators of transcription 3 in lung cancer

ZHAO Zhenshan; LI Haiyang; ZHAO Zhenxing; DAI Dai; HAO Menghui

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Medical Journal of the Chinese People Armed Police Forces ›› 2019, Vol. 30 ›› Issue (5) : 402-405.

Downregulation of p53 promotes cell proliferation and migration and inhibits apoptosis by targeting signal transducers and activators of transcription 3 in lung cancer

ZHAO Zhenshan, LI Haiyang, ZHAO Zhenxing, DAI Dai, HAO Menghui

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Abstract

Objective To investigate the expression of p53 in lung cancer and its effect on proliferation and apoptosis of lung cancer cells by regulating signal transducers and activators of transcription 3 (STAT3) signaling pathway.Methods Cells were divided into blank control group, negative control group, p53 small interference RNA (siRNA) group and p53 siRNA+STAT3 siRNA group. The expressions of p53 and STAT3 were detected by Western blotting. Transwell assay was used to measure cell migration and invasion, while flow cytometry using Annexin V/propidium iodide (AV-PI) double staining was adopted to detect cell apoptosis.Results The level of p53 expression in lung cancer tissues was significantly lower, while the positive rate of STAT3 expression was increased (all P<0.001). SiRNA transfected with p53 could significantly increase the expression level of STAT3 in lung cancer cells. The growth and migration ability of cells in p53 siRNA group was significantly increased, while the level of apoptosis was significantly reduced (P<0.001).Conclusion P53 shows low expressions in lung cancer, and can negatively regulate the expression of STAT3, thus inhibiting the ability of proliferation and apoptosis.

Key words

lung cancer / p53 / signal transducer and activator of transcription 3 / cell growth / cell migration / cell apoptosis

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ZHAO Zhenshan, LI Haiyang, ZHAO Zhenxing, DAI Dai, HAO Menghui. Downregulation of p53 promotes cell proliferation and migration and inhibits apoptosis by targeting signal transducers and activators of transcription 3 in lung cancer[J]. Medical Journal of the Chinese People Armed Police Forces. 2019, 30(5): 402-405

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