Determination of resveratrol and ε-viniferincontents in the stems of grape vines from different habitats

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Medical Journal of the Chinese People Armed Police Forces ›› 2019, Vol. 30 ›› Issue (7) : 599-602.

ORIGINAL ARTICLES

Determination of resveratrol and ε-viniferincontents in the stems of grape vines from different habitats

SONG Fei¹, HE Lingshuang², SUN Huageng², ZHAO Yanmin^2,3, LIU Dan⁴, FU Shan², LIU Dailin³

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Abstract

Objective To determine the contents of resveratrol and ε-viniferin in the stems of muscat grape vines grown in different areas.Methods High performance liquid chromatography was applied to determine the contents with a mobile phase that consisted of methanol-acetic acid aqueous solution by gradient elution. The wavelength of detection was 310 nm and 254 nm respectively, the flow rate was 1 mL/min, and the column temperature was 30 ℃. Results The linear relationships of resveratrol and ε-viniferin were good in the range of 1.76 to 17.6 μg and 2.75 to 27.54 μg respectively(R²=0.9999). The contents of resveratrol and ε-viniferin determined with this method ranged from 1.94 to 5.96μg/g and 8.74 to 15.41 μg/g in ten samples, while their coefficients of variation were 40.64% and 17.67%, respectively.Conclusions This method is simple, accurate and can be used to for quality control of grape vines.

Key words

stems of grape vines / high performance liquid chromatography / resveratrol / ε-viniferin

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REN Yongqiang, WEI Yajuan, CAO Yanan, HE Guanglun, LIU Hailing. Determination of resveratrol and ε-viniferincontents in the stems of grape vines from different habitats[J]. Medical Journal of the Chinese People Armed Police Forces. 2019, 30(7): 599-602

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