The influence of LPS on the expression of SPLUNC1 in Hela cells and A549 cells

CAO Liuxia; WU Jiansong; CHEN Jie

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Medical Journal of the Chinese People Armed Police Forces ›› 2019, Vol. 30 ›› Issue (9) : 752-755.

ORIGINAL ARTICLES

The influence of LPS on the expression of SPLUNC1 in Hela cells and A549 cells

CAO Liuxia, WU Jiansong, CHEN Jie

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Abstract

Objective To explore the influence of LPS on SPLUNC1 in Hela cells and A549 cells.Methods The two cell lines were cultured and divided into the control group and experimental group randomly. CCK-8 was used to study the time-response and dose-response relationship between LPS and proliferation and to screen the optimal concentration and action time of LPS in each cell line. After treatment with the optimal concentration and action time of LPS, the relative expression of SPLUNC1 was measured by immunohistochemical staining and immunofluorescence assay.Results The optimal concentration of LPS for A549 cells was 20 mg/L, and the optimal action time was 12 h. For Hela cells, the values were 40 mg/l and 12-24 h respectively. Both A549 cells and Hela cells expressed SPLUNC1. After LPS exposure,the expression of SPLUNC1 was up-regulated significantly(P＜0.05).Comparison between the two cell lines showed that after treatment with LPS the relative expression of SPLUNC1 in Hela cells was significantly higher than in A549 cells (P＜0.05), and the increment(Δ) of SPLUNC1 compared to the control in Hela cells was also significantly greater than in A549 cells(P＜0.05).Conclusions SPLUNC1 is expressed in cervical cancer. It can be a protective cell agent against lung adenocarcinoma and cervical cancer with potential clinical value.

Key words

SPLUNC1(short palate, lung and nasal epithelium clone 1) / A549 cells / Hela cells / neoplasm / pathogenesis

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CAO Liuxia, WU Jiansong, CHEN Jie. The influence of LPS on the expression of SPLUNC1 in Hela cells and A549 cells[J]. Medical Journal of the Chinese People Armed Police Forces. 2019, 30(9): 752-755

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