Over-expression of MiR-26b-5p inhibits HMGA1 expression and participates in epithelial-mesenchymal transformation of ovarian cancer cells

CAO Wenhong

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Medical Journal of the Chinese People Armed Police Forces ›› 2019, Vol. 30 ›› Issue (9) : 760-763.

Over-expression of MiR-26b-5p inhibits HMGA1 expression and participates in epithelial-mesenchymal transformation of ovarian cancer cells

CAO Wenhong

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Abstract

Objective To explore the mechanism by which microRNA-26b-5p (miR-26b-5p) mediates high-mobility group A1 (HMGA1) in epithelial-mesenchymal transformation of ovarian cancer.Methods The target gene of microRNA-26b-5p was predicted. The expressions of miR-26b-5p and HMGA1 in tissues were detected by real-time reverse transcription PCR (qRT-PCR). After grouping and transfection of ovarian cancer cells, the expressions of mRNA and protein of relevant factors were detected by qRT-PCR and Western blot in the cells. Transwell invasion test was used to detect cell invasion and scratch test to detect migration ability, while qRT-PCR and Western blot were used to detect the expressions of E-cadherin and Vimentin genes related to epithelial-mesenchymal transformation in tumors.Results The expression of miR-26b-5p in ovarian cancer tissue decreased, while the expression of HMGA1 increased (All P＜0.05). miR-26b-5p mimic and sh-HMGA1 groups showed decreased expressions of HMGA1 and Vimentin, increased expression of E-cadherin, and reduced cell invasion and migration (All P＜0.05). Co-transfection of miR-26b-5p inhibitor and sh-HMGA1 reversed the inhibitory effect of sh-HMGA1 on epithelial-mesenchymal transformation (All P＜0.05).Conclusions Over-expression of miR-26b-5p inhibits the expressions of HMGA1 and Vimentin in ovarian cancer cells, promotes E-cadherin expression, reduces cell invasion and migration, thus inhibiting epithelial-mesenchymal transformation of ovarian cancer cells.

Key words

ovarian cancer cell / miR-26b-5p / HMGA1 / Vimentin / E-cadherin / epithelial-mesenchymal transformation

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CAO Wenhong. Over-expression of MiR-26b-5p inhibits HMGA1 expression and participates in epithelial-mesenchymal transformation of ovarian cancer cells[J]. Medical Journal of the Chinese People Armed Police Forces. 2019, 30(9): 760-763

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