Clinical significance of miR-551b expression in cholangiocarcinoma and bioinformatics analysis of its target gene

LIU Xueting; CAI Jun; SUN Yanjun; SUN Dengqun

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Medical Journal of the Chinese People Armed Police Forces ›› 2020, Vol. 31 ›› Issue (11) : 964-968.

Clinical significance of miR-551b expression in cholangiocarcinoma and bioinformatics analysis of its target gene

LIU Xueting¹, CAI Jun¹, SUN Yanjun², SUN Dengqun²

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Abstract

Objective To study the clinical significance of miR-551b expressions in cholangiocarcinoma and analyze the target gene of mir-551b by bioinformatics.Methods Seventy-eight patients with cholangiocarcinoma who underwent radical resection in our hospital between October 2014 and October 2018 were selected as the cholangiocarcinoma group while another 40 patients who underwent Roux-en-Y cholangiojejunostomy due to bile duct stones were selected as the control group.The expressions of miR-551 and cyclin D1 in cholangiocarcinoma and normal bile duct tissues were calculated and the overall survival (OS) of patients with cholangiocarcinoma was followed up.Results The expression of miR-551b in cholangiocarcinoma tissues was lower than that in normal bile duct tissues.The expression of miR-551b in cholangiocarcinoma tissues of patients with increased preoperative serum CA19-9 and CEA,in TNM stage Ⅲ,or with low-undifferentiated and microvascular invasion were significantly lower than that of patients with normal preoperative serum CA19-9 and CEA,in TNM stageⅠ-Ⅱ,or with medium-high differentiation and non-microvascular invasion.Compared with cholangiocarcinoma patients with high miR-551b expressions,the overall survival of patients with low expressions of miR-551b was significantly shortened.miR-551b targeted 1456-1462 and 1479-1486 bases of mRNA 3′UTR of CyclinD1 gene,and the expression level of CyclinD1 in cholangiocarcinoma was significantly higher than that in the normal bile duct and negatively correlated with the expression of miR-551b.Preoperative CA19-9,TNM stage,microvascular invasion,and expressions of miR-551b and cyclinD1 were factors that influenced the overall survival of patients with cholangiocarcinoma.Conclusions The decrease of miR-551 expression in cholangiocarcinoma is related to the deterioration of pathological features and the shortening of total survival time.Targeting CyclinD1 is a possible mechanism by which miR-551 is involved in the occurrence and development of cholangiocarcinoma.

Key words

cholangiocarcinoma / mir-551b / cyclinD1 / target gene / bioinformatics

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LIU Xueting, CAI Jun, SUN Yanjun, SUN Dengqun. Clinical significance of miR-551b expression in cholangiocarcinoma and bioinformatics analysis of its target gene[J]. Medical Journal of the Chinese People Armed Police Forces. 2020, 31(11): 964-968

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